TY - JOUR
T1 - Distinct transcriptional profiles characterize bone microenvironment mesenchymal cells rather than osteoblasts in relationship with multiple myeloma bone disease
AU - Todoerti, Katia
AU - Lisignoli, Gina
AU - Storti, Paola
AU - Agnelli, Luca
AU - Novara, Francesca
AU - Manferdini, Cristina
AU - Codeluppi, Katia
AU - Colla, Simona
AU - Crugnola, Monica
AU - Abeltino, Manuela
AU - Bolzoni, Marina
AU - Sgobba, Valentina
AU - Facchini, Andrea
AU - Lambertenghi-Deliliers, Giorgio
AU - Zuffardi, Orsetta
AU - Rizzoli, Vittorio
AU - Neri, Antonino
AU - Giuliani, Nicola
N1 - Funding Information:
This article was supported by grants from Progetti Regione-Università Emilia-Romagna (Italy), the Italian Ministry of Health (Italy) (to N.G. and V.R), the Associazione Italiana Ricerca sul Cancro (Italy) (to A.N.), the Italian Ministry of Universities (MIUR) (Milan, Italy), Progetto PRIN 2006 (Italy), and the Associazione Italiana Contro le Leucemie (AIL) (Parma, Italy). K.T. and L.A. were supported by a fellowship from the Fondazione Italiana Ricerca sul Cancro (Milan, Italy).
PY - 2010/2
Y1 - 2010/2
N2 - Objective: Multiple myeloma (MM) is characterized by a high incidence of osteolytic bone lesions, which have been previously correlated with the gene expression profiles of MM cells. The aim of this study was to investigate the transcriptional patterns of cells in the bone microenvironment and their relationships with the presence of osteolysis in MM patients. Materials and Methods: Both mesenchymal (MSC) and osteoblastic (OB) cells were isolated directly from bone biopsies of MM patients and controls to perform gene expression profiling by microarrays and real-time polymerase chain reaction on selected bone-related genes. Results: We identified a series of upregulated and downregulated genes that were differentially expressed in the MSC cells of osteolytic and nonosteolytic patients. Comparison of the osteolytic and nonosteolytic samples also showed that the MSC cells and OB had distinct transcriptional patterns. No significantly modulated genes were found in the OBs of the osteolytic and nonosteolytic patients. Conclusions: Our data suggest that the gene expression profiles of cells of the bone microenvironment are different in MM patients and controls, and that MSC cells, but not OBs, have a distinct transcriptional pattern associated with the occurrence of bone lesions in MM patients. These data support the idea that alterations in MSC cells may be involved in MM bone disease.
AB - Objective: Multiple myeloma (MM) is characterized by a high incidence of osteolytic bone lesions, which have been previously correlated with the gene expression profiles of MM cells. The aim of this study was to investigate the transcriptional patterns of cells in the bone microenvironment and their relationships with the presence of osteolysis in MM patients. Materials and Methods: Both mesenchymal (MSC) and osteoblastic (OB) cells were isolated directly from bone biopsies of MM patients and controls to perform gene expression profiling by microarrays and real-time polymerase chain reaction on selected bone-related genes. Results: We identified a series of upregulated and downregulated genes that were differentially expressed in the MSC cells of osteolytic and nonosteolytic patients. Comparison of the osteolytic and nonosteolytic samples also showed that the MSC cells and OB had distinct transcriptional patterns. No significantly modulated genes were found in the OBs of the osteolytic and nonosteolytic patients. Conclusions: Our data suggest that the gene expression profiles of cells of the bone microenvironment are different in MM patients and controls, and that MSC cells, but not OBs, have a distinct transcriptional pattern associated with the occurrence of bone lesions in MM patients. These data support the idea that alterations in MSC cells may be involved in MM bone disease.
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U2 - 10.1016/j.exphem.2009.11.009
DO - 10.1016/j.exphem.2009.11.009
M3 - Article
C2 - 19963035
AN - SCOPUS:74249093697
SN - 0301-472X
VL - 38
SP - 141
EP - 153
JO - Experimental Hematology
JF - Experimental Hematology
IS - 2
ER -