TY - JOUR
T1 - Distinct WNT pathways regulating AER formation and dorsoventral polarity in the chick limb bud
AU - Kengaku, Mineko
AU - Capdevila, Javier
AU - Rodriguez-Esteban, Concepción
AU - De La Peña, Jennifer
AU - Johnson, Randy L.
AU - Belmonte, Juan Carlos Izpisúa
AU - Tabin, Clifford J.
PY - 1998/5/22
Y1 - 1998/5/22
N2 - The apical ectodermal ridge (AER) is an essential structure for vertebrate limb development. Wnt3a is expressed during the induction of the chick AER, and misexpression of Wnt3a induces ectopic expression of AER-specific genes in the limb ectoderm. The genes β-catenin and Lef1 can mimic the effect of Wnt3a, and blocking the intrinsic Lef1 activity disrupts AER formation. Hence, Wnt3a functions in AER formation through the β-catenin/LEF1 pathway. In contrast, neither β-catenin nor Lef1 affects the Wnt7a-regulated dorsoventral polarity of the limb. Thus, two related Wnt genes elicit distinct responses in the same tissues by using different intracellular pathways.
AB - The apical ectodermal ridge (AER) is an essential structure for vertebrate limb development. Wnt3a is expressed during the induction of the chick AER, and misexpression of Wnt3a induces ectopic expression of AER-specific genes in the limb ectoderm. The genes β-catenin and Lef1 can mimic the effect of Wnt3a, and blocking the intrinsic Lef1 activity disrupts AER formation. Hence, Wnt3a functions in AER formation through the β-catenin/LEF1 pathway. In contrast, neither β-catenin nor Lef1 affects the Wnt7a-regulated dorsoventral polarity of the limb. Thus, two related Wnt genes elicit distinct responses in the same tissues by using different intracellular pathways.
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U2 - 10.1126/science.280.5367.1274
DO - 10.1126/science.280.5367.1274
M3 - Article
C2 - 9596583
AN - SCOPUS:0032557302
SN - 0036-8075
VL - 280
SP - 1274
EP - 1277
JO - Science
JF - Science
IS - 5367
ER -