TY - JOUR
T1 - Diurnal variation of plasma extracellular vesicle is disrupted in people living with hiv
AU - Bazié, Wilfried Wenceslas
AU - Goyer, Benjamin
AU - Boucher, Julien
AU - Zhang, Yuwei
AU - Planas, Delphine
AU - Chatterjee, Debashree
AU - Routy, Jean Pierre
AU - Alary, Michel
AU - Ancuta, Petronela
AU - Gilbert, Caroline
N1 - Funding Information:
This research was funded through Canadian Institutes of Health Research (CIHR) grants MOP-188726; MOP-267056 (HIV/AIDS initiative) to C.G., and CIHR PJT-153052 to P.A., and in part by the Fonds de la Recherche Québec-Santé (FRQS): Réseau SIDA/Maladies infectieuses and Thérapie cellulaire; the Canadian Institutes of Health Research (CIHR; grants HOP 103230 and PTJ 166049 to JPR and CIHR Foundation Grant FDN-143218 to M.A. for the studentship awarded to W.W.B. W.W.B is the recipient of the leadership and sustainable development scholarship, W.W.B and J.B. are recipients of the recruitment Scholarship from the AIDS Research Fund of Laval University, and the Desjardins scholarship from the Foundation du CHU de Québec. J.-P.R. holds the Louis Lowenstein chair in Hematology & Oncology at McGill University. The FRQ-S supports the Centre de recherche du CHU de Québec–Université Laval’s infrastructure.
Funding Information:
Funding: This research was funded through Canadian Institutes of Health Research (CIHR) grants MOP-188726; MOP-267056 (HIV/AIDS initiative) to C.G., and CIHR PJT-153052 to P.A., and in part by the Fonds de la Recherche Québec-Santé (FRQ-S): Réseau SIDA/Maladies infectieuses and Thérapie cellulaire; the Canadian Institutes of Health Research (CIHR; grants HOP 103230 and PTJ 166049 to JPR and CIHR Foundation Grant FDN-143218 to M.A. for the studentship awarded to W.W.B. W.W.B is the recipient of the leadership and sustainable development scholarship, W.W.B and J.B. are recipients of the recruitment Scholarship from the AIDS Research Fund of Laval University, and the Desjardins scholarship from the Foundation du CHU de Québec. J.-P.R. holds the Louis Lowenstein chair in Hematology & Oncology at McGill University. The FRQ-S supports the Centre de recherche du CHU de Québec–Université Laval’s infrastructure.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - Background: Several types of extracellular vesicles (EVs) secreted by various immune and non-immune cells are present in the human plasma. We previously demonstrated that EV abundance and microRNA content change in pathological conditions, such as HIV infection. Here, we investigated daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV−). Methods: Venous blood samples from n = 10 HIV+ART and n = 10 HIV− participants were collected at 10:00 and 22:00 the same day. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-29b, miR-92, miR-155, and miR-223 copies were measured by RT-PCR. Results: Large EVs were significantly bigger in the plasma collected at 10:00 versus 22:00 in both groups. There was a significant day–night increase in the quantity of 5 miRNAs in HIV− large EVs. In HIV+ART, only miR-155 daily variation has been observed in large EVs. Finally, EV-miRNA content permits to distinguish HIV− to HIV+ART in multivariate analysis. Conclusion: These results point that plasma EV amount and microRNA contents are under daily variation in HIV− people. This new dynamic measure is disrupted in PLWH despite viral-suppressive ART. This study highlights a significant difference concerning EV abundance and their content measured at 22:00 between both groups. Therefore, the time of blood collection must be considered in the future for the EV as biomarkers.
AB - Background: Several types of extracellular vesicles (EVs) secreted by various immune and non-immune cells are present in the human plasma. We previously demonstrated that EV abundance and microRNA content change in pathological conditions, such as HIV infection. Here, we investigated daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV−). Methods: Venous blood samples from n = 10 HIV+ART and n = 10 HIV− participants were collected at 10:00 and 22:00 the same day. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-29b, miR-92, miR-155, and miR-223 copies were measured by RT-PCR. Results: Large EVs were significantly bigger in the plasma collected at 10:00 versus 22:00 in both groups. There was a significant day–night increase in the quantity of 5 miRNAs in HIV− large EVs. In HIV+ART, only miR-155 daily variation has been observed in large EVs. Finally, EV-miRNA content permits to distinguish HIV− to HIV+ART in multivariate analysis. Conclusion: These results point that plasma EV amount and microRNA contents are under daily variation in HIV− people. This new dynamic measure is disrupted in PLWH despite viral-suppressive ART. This study highlights a significant difference concerning EV abundance and their content measured at 22:00 between both groups. Therefore, the time of blood collection must be considered in the future for the EV as biomarkers.
KW - Circadian clock
KW - Extracellular vesicles
KW - HIV−1
KW - MicroRNA
KW - MiR-155
KW - MiR-223
KW - MiR-29a
KW - MiR-29b
KW - MiR-92
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U2 - 10.3390/pathogens10050518
DO - 10.3390/pathogens10050518
M3 - Article
C2 - 33923310
AN - SCOPUS:85105779531
SN - 2076-0817
VL - 10
JO - Pathogens
JF - Pathogens
IS - 5
M1 - 518
ER -