Early placenta insulin-like growth factor (pro-EPIL) is overexpressed and secreted by c-erbB-2-positive cells with high invasion potential

Burkhard Brandt; Katrin Schier; Jan Hendrik Mikesch; Dirk Kemming; Werner Boecker; Horst Buerger; Antje Roetger; Jean Michel Bidart; Jens Packeisen; Dihua Yu

Early placenta insulin-like growth factor (pro-EPIL) is overexpressed and secreted by c-erbB-2-positive cells with high invasion potential

Burkhard Brandt, Katrin Schier, Jan Hendrik Mikesch, Dirk Kemming, Werner Boecker, Horst Buerger, Antje Roetger, Jean Michel Bidart, Jens Packeisen, Dihua Yu

Molecular & Cellular Oncology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

By differential-display PCR a subclone of the SKBR3 cell line with high in vitro transendothelial invasiveness was identified to express increased levels of the INSL-4 gene. This new member of the insulin-like growth factor family encodes for a peptide, designated early placenta insulin-like (EPIL), being expressed in the so-called "invasive" phase of the placental development. Immunohistochemistry on tissue microarrays revealed a heterogeneous expression of EPIL in breast cancer tissue and no expression in the surrounding stroma cells. A coexpression of pro-EPIL and c-erbB-2 could be observed predominantly in cell clusters at the infiltrating edge of the tumor. Our results give new suggestions for the presence of a signaling network of receptor tyrosine kinases underlying breast cancer invasion and metastasis.

Original language	English (US)
Pages (from-to)	1020-1024
Number of pages	5
Journal	Cancer Research
Volume	62
Issue number	4
State	Published - Feb 15 2002

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Early placenta insulin-like growth factor (pro-EPIL) is overexpressed and secreted by c-erbB-2-positive cells with high invasion potential",

abstract = "By differential-display PCR a subclone of the SKBR3 cell line with high in vitro transendothelial invasiveness was identified to express increased levels of the INSL-4 gene. This new member of the insulin-like growth factor family encodes for a peptide, designated early placenta insulin-like (EPIL), being expressed in the so-called {"}invasive{"} phase of the placental development. Immunohistochemistry on tissue microarrays revealed a heterogeneous expression of EPIL in breast cancer tissue and no expression in the surrounding stroma cells. A coexpression of pro-EPIL and c-erbB-2 could be observed predominantly in cell clusters at the infiltrating edge of the tumor. Our results give new suggestions for the presence of a signaling network of receptor tyrosine kinases underlying breast cancer invasion and metastasis.",

author = "Burkhard Brandt and Katrin Schier and Mikesch, {Jan Hendrik} and Dirk Kemming and Werner Boecker and Horst Buerger and Antje Roetger and Bidart, {Jean Michel} and Jens Packeisen and Dihua Yu",

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T1 - Early placenta insulin-like growth factor (pro-EPIL) is overexpressed and secreted by c-erbB-2-positive cells with high invasion potential

AU - Brandt, Burkhard

AU - Schier, Katrin

AU - Mikesch, Jan Hendrik

AU - Kemming, Dirk

AU - Boecker, Werner

AU - Buerger, Horst

AU - Roetger, Antje

AU - Bidart, Jean Michel

AU - Packeisen, Jens

AU - Yu, Dihua

PY - 2002/2/15

Y1 - 2002/2/15

N2 - By differential-display PCR a subclone of the SKBR3 cell line with high in vitro transendothelial invasiveness was identified to express increased levels of the INSL-4 gene. This new member of the insulin-like growth factor family encodes for a peptide, designated early placenta insulin-like (EPIL), being expressed in the so-called "invasive" phase of the placental development. Immunohistochemistry on tissue microarrays revealed a heterogeneous expression of EPIL in breast cancer tissue and no expression in the surrounding stroma cells. A coexpression of pro-EPIL and c-erbB-2 could be observed predominantly in cell clusters at the infiltrating edge of the tumor. Our results give new suggestions for the presence of a signaling network of receptor tyrosine kinases underlying breast cancer invasion and metastasis.

AB - By differential-display PCR a subclone of the SKBR3 cell line with high in vitro transendothelial invasiveness was identified to express increased levels of the INSL-4 gene. This new member of the insulin-like growth factor family encodes for a peptide, designated early placenta insulin-like (EPIL), being expressed in the so-called "invasive" phase of the placental development. Immunohistochemistry on tissue microarrays revealed a heterogeneous expression of EPIL in breast cancer tissue and no expression in the surrounding stroma cells. A coexpression of pro-EPIL and c-erbB-2 could be observed predominantly in cell clusters at the infiltrating edge of the tumor. Our results give new suggestions for the presence of a signaling network of receptor tyrosine kinases underlying breast cancer invasion and metastasis.

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Early placenta insulin-like growth factor (pro-EPIL) is overexpressed and secreted by c-erbB-2-positive cells with high invasion potential

Abstract

ASJC Scopus subject areas

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