TY - JOUR
T1 - Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells
T2 - Cytogenetic analysis of the stem cell subpopulation
AU - Podhajcer, Osvaldo L.
AU - Resnicoff, Mariana
AU - Bover, Laura
AU - Medrano, Estela E.
AU - Slavutsky, Irma
AU - Larripa, Irene
AU - Mordoh, Jose
N1 - Funding Information:
This work has been supported by Grants from the Consejo National de Investigaciones Cientificas y Tecnicas (CONICET), the University of Buenos Aires and the Comisi6n de Investigaciones Cientilicas de la Provincia de Buenos Aires (CIC). L. B., E. E. M., I. L., I. S. and J. M. are members of the CONICET, 0. L. P. is member of the CIC, and M. R. is a Fellow of the Fundaci6n Maria Calderon de la Barca.
PY - 1988/11
Y1 - 1988/11
N2 - The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone.
AB - The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone.
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U2 - 10.1016/0014-4827(88)90348-5
DO - 10.1016/0014-4827(88)90348-5
M3 - Article
C2 - 3169151
AN - SCOPUS:0023700205
SN - 0014-4827
VL - 179
SP - 58
EP - 64
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -