Effect of neuraminidase on the rat one-way mixed lymphocyte interaction

Isaiah J. Fidler; Jeffrey M. Kahn; Paul C. Montgomery

doi:10.3109/08820137309022828

Effect of neuraminidase on the rat one-way mixed lymphocyte interaction

Isaiah J. Fidler, Jeffrey M. Kahn, Paul C. Montgomery

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3 Scopus citations

Abstract

The effect of Vibrio cholerae neuraminidase (VCN) on the rat one-way mixed leukocyte interaction (MLI) was investigated. VCN was iodinated without apparent loss of biological activity. Treatment of stimulatory cells with either cold or ¹²⁵I-labeled VCN significantly enhanced their subsequent reactivity in the MLI. In contrast, treatment of the responding cells alone did not increase the response. Our studies rule out the possibility that VCN increases cellular immunogenicity by binding to the lymphocyte surface. The mechanism of VCN action appears to involve a direct surface modification of lymphoid cells; this modification leads to their increased reactivity.

Original language	English (US)
Pages (from-to)	573-583
Number of pages	11
Journal	Immunological Investigations
Volume	2
Issue number	6
DOIs	https://doi.org/10.3109/08820137309022828
State	Published - 1973
Externally published	Yes

ASJC Scopus subject areas

Immunology

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title = "Effect of neuraminidase on the rat one-way mixed lymphocyte interaction",

abstract = "The effect of Vibrio cholerae neuraminidase (VCN) on the rat one-way mixed leukocyte interaction (MLI) was investigated. VCN was iodinated without apparent loss of biological activity. Treatment of stimulatory cells with either cold or 125I-labeled VCN significantly enhanced their subsequent reactivity in the MLI. In contrast, treatment of the responding cells alone did not increase the response. Our studies rule out the possibility that VCN increases cellular immunogenicity by binding to the lymphocyte surface. The mechanism of VCN action appears to involve a direct surface modification of lymphoid cells; this modification leads to their increased reactivity.",

author = "Fidler, {Isaiah J.} and Kahn, {Jeffrey M.} and Montgomery, {Paul C.}",

year = "1973",

doi = "10.3109/08820137309022828",

language = "English (US)",

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pages = "573--583",

journal = "Immunological Investigations",

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T1 - Effect of neuraminidase on the rat one-way mixed lymphocyte interaction

AU - Fidler, Isaiah J.

AU - Kahn, Jeffrey M.

AU - Montgomery, Paul C.

PY - 1973

Y1 - 1973

N2 - The effect of Vibrio cholerae neuraminidase (VCN) on the rat one-way mixed leukocyte interaction (MLI) was investigated. VCN was iodinated without apparent loss of biological activity. Treatment of stimulatory cells with either cold or 125I-labeled VCN significantly enhanced their subsequent reactivity in the MLI. In contrast, treatment of the responding cells alone did not increase the response. Our studies rule out the possibility that VCN increases cellular immunogenicity by binding to the lymphocyte surface. The mechanism of VCN action appears to involve a direct surface modification of lymphoid cells; this modification leads to their increased reactivity.

AB - The effect of Vibrio cholerae neuraminidase (VCN) on the rat one-way mixed leukocyte interaction (MLI) was investigated. VCN was iodinated without apparent loss of biological activity. Treatment of stimulatory cells with either cold or 125I-labeled VCN significantly enhanced their subsequent reactivity in the MLI. In contrast, treatment of the responding cells alone did not increase the response. Our studies rule out the possibility that VCN increases cellular immunogenicity by binding to the lymphocyte surface. The mechanism of VCN action appears to involve a direct surface modification of lymphoid cells; this modification leads to their increased reactivity.

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SP - 573

EP - 583

JO - Immunological Investigations

JF - Immunological Investigations

IS - 6

ER -

Effect of neuraminidase on the rat one-way mixed lymphocyte interaction

Abstract

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