TY - JOUR
T1 - Effects of extracellular matrix on the response of endothelial cells to radiation in vitro
AU - Fuks, Zvi
AU - Vlodavsky, Israel
AU - Andreeff, Michael
AU - McLoughlin, Maureen
AU - Haimovitz-Friedman, Adriana
N1 - Funding Information:
Acknowledgements-We thank Mr Roger S. Persaud and Mr L.God-frey for their excellent technical assistance.T he work described in this paper was supported by NIH Grant CA-52462.
PY - 1992
Y1 - 1992
N2 - The study demonstrates that the sensitivity of endothelial cells to irradiation in vitro is significantly affected by the microenvironmental conditions under which the experiment is carried out. When irradiated plateau phase bovine aortic endothelial cells were assayed for colony formation on top of uncoated plastic of standard culture dishes, the dose survival curves showed Do values of [mean (S.D.)] 107 (6) cGy and Dq of 63 (28) cGy (calculated according to the single-hit multitarget model). When assayed in dishes precoated with the autologous natural basement membrane-like extracellular matrix ( BAEC ECM) the curves showed a similar Do [106 (2) cGy], but the Dq was 194 (8) cGy (P < 0.05), indicating that components of natural ECM confer in endothelial cells an improved capacity to repair radiation lesions and to restore the clonogenic capacity. However, when the natural but biologically unrelated HR9-bFGF ECM was used, a decreased repair capacity was noted with Dq of 156 (30) cGy (P < 0.05 compared with BAEC ECM). The data demonstrate the high specificity of the repair function to interactions with autologous matrix components, and emphasise the need to select relevant experimental conditions when parameters of the radiation response in vitro are used to predict the response in vivo.
AB - The study demonstrates that the sensitivity of endothelial cells to irradiation in vitro is significantly affected by the microenvironmental conditions under which the experiment is carried out. When irradiated plateau phase bovine aortic endothelial cells were assayed for colony formation on top of uncoated plastic of standard culture dishes, the dose survival curves showed Do values of [mean (S.D.)] 107 (6) cGy and Dq of 63 (28) cGy (calculated according to the single-hit multitarget model). When assayed in dishes precoated with the autologous natural basement membrane-like extracellular matrix ( BAEC ECM) the curves showed a similar Do [106 (2) cGy], but the Dq was 194 (8) cGy (P < 0.05), indicating that components of natural ECM confer in endothelial cells an improved capacity to repair radiation lesions and to restore the clonogenic capacity. However, when the natural but biologically unrelated HR9-bFGF ECM was used, a decreased repair capacity was noted with Dq of 156 (30) cGy (P < 0.05 compared with BAEC ECM). The data demonstrate the high specificity of the repair function to interactions with autologous matrix components, and emphasise the need to select relevant experimental conditions when parameters of the radiation response in vitro are used to predict the response in vivo.
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U2 - 10.1016/0959-8049(92)90104-A
DO - 10.1016/0959-8049(92)90104-A
M3 - Article
C2 - 1524892
AN - SCOPUS:0026605347
SN - 0959-8049
VL - 28
SP - 725
EP - 731
JO - European Journal of Cancer
JF - European Journal of Cancer
IS - 4-5
ER -