Effects of holmium:YAG laser energy on cartilage metabolism, healing, and biochemical properties of lesional and perilesional tissue in a weight-bearing model

J. Gregory Pullin; Michael A. Collier; Prajnan Das; R. L. Smith; Lawrence E. DeBault; Lanny L. Johnson; Robert C. Walls

doi:10.1016/S0749-8063(96)90214-5

Effects of holmium:YAG laser energy on cartilage metabolism, healing, and biochemical properties of lesional and perilesional tissue in a weight-bearing model

J. Gregory Pullin, Michael A. Collier, Prajnan Das, R. L. Smith, Lawrence E. DeBault, Lanny L. Johnson, Robert C. Walls

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Comparison of perilesional cartilage, lesional repair tissue, and subchondral bone activity 6 months after application of holmium-yttrium- aluminum-garnet (Ho:YAG) laser energy to chronic (10 week) induced 10-mm full-thickness (FT) circular articular cartilage craters followed by 6 months' intermittent active motion (IAM) in a free exercise environment was investigated. The 2.1-μm wavelength was delivered in hand-controlled near-contact mode by arthroscopic surgery in a saline medium. Bilateral arthroscopy was performed on normal antebrachiocarpal, intercarpal, and metacarpophalangeal joints of six adult horses. Full-thickness craters were created in nine sites per limb on weight-bearing articular surfaces with a motorized bur. Right limb craters served as sham operated controls. Animals were killed at 10 weeks after FT crater creation (n = 2), and at 24 weeks (6 months) after laser energy application (n = 4). Histological analysis using hematoxylineosin (HE) and Safranin-O staining consisted of a modified Mankin grading of perilesional cartilage and lesional repair tissue scoring. Biochemical analysis was performed for cellularity and glycosaminoglycan (GAG) synthesis. Histological analysis showed clustering of chondrocytes or perilesional zonal cloning (PZC) in 83% of laser-treated lesions and in no control lesions. No differences were observed between treated and control lesional repair activity. Laser-treated perilesional cartilage showed a significant (P < .02) decrease in GAG synthesis. No adverse effects to distant cartilage were observed after application of laser energy regarding cell proliferation or GAG synthesis. Significance of decreased GAG synthesis in treated perilesional cartilage and perilesional zonal cloning of chondrocytes in treated cartilage is unknown. Additional study of Ho:YAG laser energy application to cartilage and subchondral bone is needed before its application in the surgical management and repair of cartilage damage.

Original language	English (US)
Pages (from-to)	15-25
Number of pages	11
Journal	Arthroscopy - Journal of Arthroscopic and Related Surgery
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/S0749-8063(96)90214-5
State	Published - 1996
Externally published	Yes

Keywords

Cartilage
Cartilage biochemistry
Clones
Healing response
Ho:YAG laser

ASJC Scopus subject areas

Orthopedics and Sports Medicine

Access to Document

10.1016/S0749-8063(96)90214-5

Cite this

Pullin, J. G., Collier, M. A., Das, P., Smith, R. L., DeBault, L. E., Johnson, L. L., & Walls, R. C. (1996). Effects of holmium:YAG laser energy on cartilage metabolism, healing, and biochemical properties of lesional and perilesional tissue in a weight-bearing model. Arthroscopy - Journal of Arthroscopic and Related Surgery, 12(1), 15-25. https://doi.org/10.1016/S0749-8063(96)90214-5

Effects of holmium:YAG laser energy on cartilage metabolism, healing, and biochemical properties of lesional and perilesional tissue in a weight-bearing model. / Pullin, J. Gregory; Collier, Michael A.; Das, Prajnan et al.
In: Arthroscopy - Journal of Arthroscopic and Related Surgery, Vol. 12, No. 1, 1996, p. 15-25.

Research output: Contribution to journal › Article › peer-review

Pullin, JG, Collier, MA, Das, P, Smith, RL, DeBault, LE, Johnson, LL & Walls, RC 1996, 'Effects of holmium:YAG laser energy on cartilage metabolism, healing, and biochemical properties of lesional and perilesional tissue in a weight-bearing model', Arthroscopy - Journal of Arthroscopic and Related Surgery, vol. 12, no. 1, pp. 15-25. https://doi.org/10.1016/S0749-8063(96)90214-5

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title = "Effects of holmium:YAG laser energy on cartilage metabolism, healing, and biochemical properties of lesional and perilesional tissue in a weight-bearing model",

abstract = "Comparison of perilesional cartilage, lesional repair tissue, and subchondral bone activity 6 months after application of holmium-yttrium- aluminum-garnet (Ho:YAG) laser energy to chronic (10 week) induced 10-mm full-thickness (FT) circular articular cartilage craters followed by 6 months' intermittent active motion (IAM) in a free exercise environment was investigated. The 2.1-μm wavelength was delivered in hand-controlled near-contact mode by arthroscopic surgery in a saline medium. Bilateral arthroscopy was performed on normal antebrachiocarpal, intercarpal, and metacarpophalangeal joints of six adult horses. Full-thickness craters were created in nine sites per limb on weight-bearing articular surfaces with a motorized bur. Right limb craters served as sham operated controls. Animals were killed at 10 weeks after FT crater creation (n = 2), and at 24 weeks (6 months) after laser energy application (n = 4). Histological analysis using hematoxylineosin (HE) and Safranin-O staining consisted of a modified Mankin grading of perilesional cartilage and lesional repair tissue scoring. Biochemical analysis was performed for cellularity and glycosaminoglycan (GAG) synthesis. Histological analysis showed clustering of chondrocytes or perilesional zonal cloning (PZC) in 83% of laser-treated lesions and in no control lesions. No differences were observed between treated and control lesional repair activity. Laser-treated perilesional cartilage showed a significant (P < .02) decrease in GAG synthesis. No adverse effects to distant cartilage were observed after application of laser energy regarding cell proliferation or GAG synthesis. Significance of decreased GAG synthesis in treated perilesional cartilage and perilesional zonal cloning of chondrocytes in treated cartilage is unknown. Additional study of Ho:YAG laser energy application to cartilage and subchondral bone is needed before its application in the surgical management and repair of cartilage damage.",

keywords = "Cartilage, Cartilage biochemistry, Clones, Healing response, Ho:YAG laser",

author = "Pullin, {J. Gregory} and Collier, {Michael A.} and Prajnan Das and Smith, {R. L.} and DeBault, {Lawrence E.} and Johnson, {Lanny L.} and Walls, {Robert C.}",

note = "Funding Information: Acknowledgment: Supported by Grants from the Department of the Navy ONR (N000014-90-C-0029) and NIH LAMP Resource Grant No. 01192; Coherent Laser Inc., Palo Alto, California; Dr. Lanny L. Johnson, M.D., Research Fund, East Lansing, Michigan; Beckman Laser Institute, Ir-vine, California; and Jules & Doris Stein Foundation, Bever-ley Hills, California. Equipment and technical support was provided by Smith Nephew Dyonics, Inc., Andover, Massachusetts, Facilities support was provided by Oklahoma State University, Stillwater, Oklahoma, U.S.A. Appreciation is extended to Martha Newman, Dr Steven Arnoczky, and Howard Doughty for technical assistance and Chris Durham for manuscript development.",

year = "1996",

doi = "10.1016/S0749-8063(96)90214-5",

language = "English (US)",

volume = "12",

pages = "15--25",

journal = "Arthroscopy - Journal of Arthroscopic and Related Surgery",

issn = "0749-8063",

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T1 - Effects of holmium:YAG laser energy on cartilage metabolism, healing, and biochemical properties of lesional and perilesional tissue in a weight-bearing model

AU - Pullin, J. Gregory

AU - Collier, Michael A.

AU - Das, Prajnan

AU - Smith, R. L.

AU - DeBault, Lawrence E.

AU - Johnson, Lanny L.

AU - Walls, Robert C.

N1 - Funding Information: Acknowledgment: Supported by Grants from the Department of the Navy ONR (N000014-90-C-0029) and NIH LAMP Resource Grant No. 01192; Coherent Laser Inc., Palo Alto, California; Dr. Lanny L. Johnson, M.D., Research Fund, East Lansing, Michigan; Beckman Laser Institute, Ir-vine, California; and Jules & Doris Stein Foundation, Bever-ley Hills, California. Equipment and technical support was provided by Smith Nephew Dyonics, Inc., Andover, Massachusetts, Facilities support was provided by Oklahoma State University, Stillwater, Oklahoma, U.S.A. Appreciation is extended to Martha Newman, Dr Steven Arnoczky, and Howard Doughty for technical assistance and Chris Durham for manuscript development.

PY - 1996

Y1 - 1996

N2 - Comparison of perilesional cartilage, lesional repair tissue, and subchondral bone activity 6 months after application of holmium-yttrium- aluminum-garnet (Ho:YAG) laser energy to chronic (10 week) induced 10-mm full-thickness (FT) circular articular cartilage craters followed by 6 months' intermittent active motion (IAM) in a free exercise environment was investigated. The 2.1-μm wavelength was delivered in hand-controlled near-contact mode by arthroscopic surgery in a saline medium. Bilateral arthroscopy was performed on normal antebrachiocarpal, intercarpal, and metacarpophalangeal joints of six adult horses. Full-thickness craters were created in nine sites per limb on weight-bearing articular surfaces with a motorized bur. Right limb craters served as sham operated controls. Animals were killed at 10 weeks after FT crater creation (n = 2), and at 24 weeks (6 months) after laser energy application (n = 4). Histological analysis using hematoxylineosin (HE) and Safranin-O staining consisted of a modified Mankin grading of perilesional cartilage and lesional repair tissue scoring. Biochemical analysis was performed for cellularity and glycosaminoglycan (GAG) synthesis. Histological analysis showed clustering of chondrocytes or perilesional zonal cloning (PZC) in 83% of laser-treated lesions and in no control lesions. No differences were observed between treated and control lesional repair activity. Laser-treated perilesional cartilage showed a significant (P < .02) decrease in GAG synthesis. No adverse effects to distant cartilage were observed after application of laser energy regarding cell proliferation or GAG synthesis. Significance of decreased GAG synthesis in treated perilesional cartilage and perilesional zonal cloning of chondrocytes in treated cartilage is unknown. Additional study of Ho:YAG laser energy application to cartilage and subchondral bone is needed before its application in the surgical management and repair of cartilage damage.

AB - Comparison of perilesional cartilage, lesional repair tissue, and subchondral bone activity 6 months after application of holmium-yttrium- aluminum-garnet (Ho:YAG) laser energy to chronic (10 week) induced 10-mm full-thickness (FT) circular articular cartilage craters followed by 6 months' intermittent active motion (IAM) in a free exercise environment was investigated. The 2.1-μm wavelength was delivered in hand-controlled near-contact mode by arthroscopic surgery in a saline medium. Bilateral arthroscopy was performed on normal antebrachiocarpal, intercarpal, and metacarpophalangeal joints of six adult horses. Full-thickness craters were created in nine sites per limb on weight-bearing articular surfaces with a motorized bur. Right limb craters served as sham operated controls. Animals were killed at 10 weeks after FT crater creation (n = 2), and at 24 weeks (6 months) after laser energy application (n = 4). Histological analysis using hematoxylineosin (HE) and Safranin-O staining consisted of a modified Mankin grading of perilesional cartilage and lesional repair tissue scoring. Biochemical analysis was performed for cellularity and glycosaminoglycan (GAG) synthesis. Histological analysis showed clustering of chondrocytes or perilesional zonal cloning (PZC) in 83% of laser-treated lesions and in no control lesions. No differences were observed between treated and control lesional repair activity. Laser-treated perilesional cartilage showed a significant (P < .02) decrease in GAG synthesis. No adverse effects to distant cartilage were observed after application of laser energy regarding cell proliferation or GAG synthesis. Significance of decreased GAG synthesis in treated perilesional cartilage and perilesional zonal cloning of chondrocytes in treated cartilage is unknown. Additional study of Ho:YAG laser energy application to cartilage and subchondral bone is needed before its application in the surgical management and repair of cartilage damage.

KW - Cartilage

KW - Cartilage biochemistry

KW - Clones

KW - Healing response

KW - Ho:YAG laser

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Effects of holmium:YAG laser energy on cartilage metabolism, healing, and biochemical properties of lesional and perilesional tissue in a weight-bearing model

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Keywords

ASJC Scopus subject areas

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