Effects of light and temperature on the expression of the Lhcb2 gene in pea

Qin Miao Sun; Liang Bi Li; Jiu Sheng Yan; Da Zhang Mao; Ting Yun Kuang

Effects of light and temperature on the expression of the Lhcb2 gene in pea

Qin Miao Sun, Liang Bi Li, Jiu Sheng Yan, Da Zhang Mao, Ting Yun Kuang

Research output: Contribution to journal › Article › peer-review

Abstract

An approximately 800 bp cDNA (Lhcb2) encoding light-harvesting chlorophyll a/b-binding protein complex (type II) was cloned from the seedling of pea (Pisum sativum L.) with RT-PCR method. Southern blotting using special probe demonstrated that there existed one copy of Lhcb2 in pea genome. RT-PCR and Northern blotting revealed the expression of Lhcb2 which was regulated by light in a time-dependent expression manner. The Lhcb2 gene didn't express untill 2 h after irradiated with white light. Low temperature (4 °C) also affected the Lhcb2 gene by decreasing half of its expression under 25 °C.

Original language	English (US)
Pages (from-to)	258-262
Number of pages	5
Journal	Acta Botanica Sinica
Volume	42
Issue number	3
State	Published - Mar 2000
Externally published	Yes

Keywords

Expression
Lhcb2 gene
Light and temperature
Pea

ASJC Scopus subject areas

Biochemistry
General Biochemistry, Genetics and Molecular Biology
Plant Science

Cite this

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title = "Effects of light and temperature on the expression of the Lhcb2 gene in pea",

abstract = "An approximately 800 bp cDNA (Lhcb2) encoding light-harvesting chlorophyll a/b-binding protein complex (type II) was cloned from the seedling of pea (Pisum sativum L.) with RT-PCR method. Southern blotting using special probe demonstrated that there existed one copy of Lhcb2 in pea genome. RT-PCR and Northern blotting revealed the expression of Lhcb2 which was regulated by light in a time-dependent expression manner. The Lhcb2 gene didn't express untill 2 h after irradiated with white light. Low temperature (4 °C) also affected the Lhcb2 gene by decreasing half of its expression under 25 °C.",

keywords = "Expression, Lhcb2 gene, Light and temperature, Pea",

author = "Sun, {Qin Miao} and Li, {Liang Bi} and Yan, {Jiu Sheng} and Mao, {Da Zhang} and Kuang, {Ting Yun}",

year = "2000",

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journal = "Acta Botanica Sinica",

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T1 - Effects of light and temperature on the expression of the Lhcb2 gene in pea

AU - Sun, Qin Miao

AU - Li, Liang Bi

AU - Yan, Jiu Sheng

AU - Mao, Da Zhang

AU - Kuang, Ting Yun

PY - 2000/3

Y1 - 2000/3

N2 - An approximately 800 bp cDNA (Lhcb2) encoding light-harvesting chlorophyll a/b-binding protein complex (type II) was cloned from the seedling of pea (Pisum sativum L.) with RT-PCR method. Southern blotting using special probe demonstrated that there existed one copy of Lhcb2 in pea genome. RT-PCR and Northern blotting revealed the expression of Lhcb2 which was regulated by light in a time-dependent expression manner. The Lhcb2 gene didn't express untill 2 h after irradiated with white light. Low temperature (4 °C) also affected the Lhcb2 gene by decreasing half of its expression under 25 °C.

AB - An approximately 800 bp cDNA (Lhcb2) encoding light-harvesting chlorophyll a/b-binding protein complex (type II) was cloned from the seedling of pea (Pisum sativum L.) with RT-PCR method. Southern blotting using special probe demonstrated that there existed one copy of Lhcb2 in pea genome. RT-PCR and Northern blotting revealed the expression of Lhcb2 which was regulated by light in a time-dependent expression manner. The Lhcb2 gene didn't express untill 2 h after irradiated with white light. Low temperature (4 °C) also affected the Lhcb2 gene by decreasing half of its expression under 25 °C.

KW - Expression

KW - Lhcb2 gene

KW - Light and temperature

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Effects of light and temperature on the expression of the Lhcb2 gene in pea

Abstract

Keywords

ASJC Scopus subject areas

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