Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

Angelika Görg; Wilhelm Postel; Johann Weser; Siegfried Günther; John R. Strahler; Samir M. Hanash; Luke Somerlot

doi:10.1002/elps.1150080207

Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

Angelika Görg, Wilhelm Postel, Johann Weser, Siegfried Günther, John R. Strahler, Samir M. Hanash, Luke Somerlot

Research output: Contribution to journal › Article › peer-review

241 Scopus citations

Abstract

Silver staining artifacts, unrelated to protein, such as horizontal lines and vertical micro or point streaking on two‐dimensional gels with carrier ampholytes or immobilized pH gradients in the first dimension are efficiently eliminated by adding iodoacetamide to the equilibration buffer of the first‐dimensional gel.

Original language	English (US)
Pages (from-to)	122-124
Number of pages	3
Journal	ELECTROPHORESIS
Volume	8
Issue number	2
DOIs	https://doi.org/10.1002/elps.1150080207
State	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.1150080207

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title = "Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer",

abstract = "Silver staining artifacts, unrelated to protein, such as horizontal lines and vertical micro or point streaking on two‐dimensional gels with carrier ampholytes or immobilized pH gradients in the first dimension are efficiently eliminated by adding iodoacetamide to the equilibration buffer of the first‐dimensional gel.",

author = "Angelika G{\"o}rg and Wilhelm Postel and Johann Weser and Siegfried G{\"u}nther and Strahler, {John R.} and Hanash, {Samir M.} and Luke Somerlot",

year = "1987",

doi = "10.1002/elps.1150080207",

language = "English (US)",

volume = "8",

pages = "122--124",

journal = "ELECTROPHORESIS",

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T1 - Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

AU - Görg, Angelika

AU - Postel, Wilhelm

AU - Weser, Johann

AU - Günther, Siegfried

AU - Strahler, John R.

AU - Hanash, Samir M.

AU - Somerlot, Luke

PY - 1987

Y1 - 1987

N2 - Silver staining artifacts, unrelated to protein, such as horizontal lines and vertical micro or point streaking on two‐dimensional gels with carrier ampholytes or immobilized pH gradients in the first dimension are efficiently eliminated by adding iodoacetamide to the equilibration buffer of the first‐dimensional gel.

AB - Silver staining artifacts, unrelated to protein, such as horizontal lines and vertical micro or point streaking on two‐dimensional gels with carrier ampholytes or immobilized pH gradients in the first dimension are efficiently eliminated by adding iodoacetamide to the equilibration buffer of the first‐dimensional gel.

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DO - 10.1002/elps.1150080207

M3 - Article

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VL - 8

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JF - ELECTROPHORESIS

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ER -

Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

Abstract

ASJC Scopus subject areas

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