TY - JOUR
T1 - Endothelial nitric oxide synthase polymorphisms and adaptation of parasympathetic modulation to exercise training
AU - Silva, Bruno M.
AU - Neves, Fabricia J.
AU - Negrão, Marcelo V.
AU - Alves, Cleber R.
AU - Dias, Rodrigo G.
AU - Alves, Guilherme B.
AU - Pereira, Alexandre C.
AU - Rondon, Maria U.
AU - Krieger, José E.
AU - Negrão, Carlos E.
AU - Lucas Da Nó Brega, Antonio Claudio
N1 - Funding Information:
This study was supported by São Paulo Research Foundation (FAPESP) 2005/59740-7, CNPq 474621/2004-9, and Fundação Zerbini. B.M.S. and F.J.N. were supported by HYPERLINK "http:// www.iie.org/en/Programs/CAPES.aspx"Coordination for the Improvement of Higher Education Personnel (CAPES) 0723-09-6 and 2690/09-8, respectively. A.C.L.N. was supported by Brazilian National Council of Scientific and Technological Development (CNPq) 307251/2009-8 and Foundation of Research Support of Rio de Janeiro State (FAPERJ) E-26/102.378/2009. M.V.N. was supported by FAPESP 2007/52784-4. M.U.R. and C.E.N. were supported by CNPq 303518/2008-1 and 302146/2007-5, respectively.
Publisher Copyright:
© Copyright 2011 by the American College of Sports Medicine.
PY - 2011/2/28
Y1 - 2011/2/28
N2 - SILVA, B. M., F. J. NEVES, M. V. NEGRA. O, C. R. ALVES, R. G. DIAS, G. B. ALVES, A. C. PEREIRA, M. U. RONDON, J. E. KRIEGER, C. E. NEGRA. O, and A. C. DA NO BREGA. Endothelial Nitric Oxide Synthase Polymorphisms and Adaptation of Parasympathetic Modulation to Exercise Training. Med. Sci. Sports Exerc., Vol. 43, No. 9, pp. 1611.1618, 2011. Purpose: There is a large interindividual variation in the parasympathetic adaptation induced by aerobic exercise training, which may be partially attributed to genetic polymorphisms. Therefore, we investigated the association among three polymorphisms in the endothelial nitric oxide gene (-786T>C, 4b4a, and 894G>T), analyzed individually and as haplotypes, and the parasympathetic adaptation induced by exercise training. Methods: Eighty healthy males, age 20.35 yr, were genotyped by polymerase chain reaction.restriction fragment length polymorphism analysis, and haplotypes were inferred using the software PHASE 2.1. Autonomic modulation (i.e., HR variability and spontaneous baroreflex sensitivity) and peak oxygen consumption (V O2peak) were measured before and after training (running, moderate to severe intensity, three times per week, 60 min·day-1, during 18 wk). Results: Training increased V O2peak (P < 0.05) and decreased mean arterial pressure (P < 0.05) in the whole sample. Subjects with the -786C polymorphic allele had a significant reduction in baroreflex sensitivity after training (change: wild type (-786TT) = 2% T 89% vs polymorphic (-786TC/CC) = -28% T 60%, median T quartile range, P = 0.03), and parasympathetic modulation was marginally reduced in subjects with the 894T polymorphic allele (change: wild type (894GG) = 8% T 67% vs polymorphic (894GT/TT) = -18% T 59%, median T quartile range, P = 0.06). Furthermore, parasympathetic modulation percent change was different between the haplotypes containing wild-type alleles (-786T/4b/894G) and polymorphic alleles at positions -786 and 894 (-786C/4b/894T) (-6% T 56% vs -41% T 50%, median T quartile range, P = 0.04). Conclusions: The polymorphic allele at position -786 and the haplotype containing polymorphic alleles at positions -786 and 894 in the endothelial nitric oxide gene were associated with decreased parasympathetic modulation after exercise training.
AB - SILVA, B. M., F. J. NEVES, M. V. NEGRA. O, C. R. ALVES, R. G. DIAS, G. B. ALVES, A. C. PEREIRA, M. U. RONDON, J. E. KRIEGER, C. E. NEGRA. O, and A. C. DA NO BREGA. Endothelial Nitric Oxide Synthase Polymorphisms and Adaptation of Parasympathetic Modulation to Exercise Training. Med. Sci. Sports Exerc., Vol. 43, No. 9, pp. 1611.1618, 2011. Purpose: There is a large interindividual variation in the parasympathetic adaptation induced by aerobic exercise training, which may be partially attributed to genetic polymorphisms. Therefore, we investigated the association among three polymorphisms in the endothelial nitric oxide gene (-786T>C, 4b4a, and 894G>T), analyzed individually and as haplotypes, and the parasympathetic adaptation induced by exercise training. Methods: Eighty healthy males, age 20.35 yr, were genotyped by polymerase chain reaction.restriction fragment length polymorphism analysis, and haplotypes were inferred using the software PHASE 2.1. Autonomic modulation (i.e., HR variability and spontaneous baroreflex sensitivity) and peak oxygen consumption (V O2peak) were measured before and after training (running, moderate to severe intensity, three times per week, 60 min·day-1, during 18 wk). Results: Training increased V O2peak (P < 0.05) and decreased mean arterial pressure (P < 0.05) in the whole sample. Subjects with the -786C polymorphic allele had a significant reduction in baroreflex sensitivity after training (change: wild type (-786TT) = 2% T 89% vs polymorphic (-786TC/CC) = -28% T 60%, median T quartile range, P = 0.03), and parasympathetic modulation was marginally reduced in subjects with the 894T polymorphic allele (change: wild type (894GG) = 8% T 67% vs polymorphic (894GT/TT) = -18% T 59%, median T quartile range, P = 0.06). Furthermore, parasympathetic modulation percent change was different between the haplotypes containing wild-type alleles (-786T/4b/894G) and polymorphic alleles at positions -786 and 894 (-786C/4b/894T) (-6% T 56% vs -41% T 50%, median T quartile range, P = 0.04). Conclusions: The polymorphic allele at position -786 and the haplotype containing polymorphic alleles at positions -786 and 894 in the endothelial nitric oxide gene were associated with decreased parasympathetic modulation after exercise training.
KW - Autonomic nervous system
KW - Genetic variation
KW - NOS3
KW - Physical training
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U2 - 10.1249/MSS.0b013e3182152197
DO - 10.1249/MSS.0b013e3182152197
M3 - Article
C2 - 21364486
AN - SCOPUS:79952081886
SN - 0195-9131
VL - 43
SP - 1611
EP - 1618
JO - Medicine and science in sports and exercise
JF - Medicine and science in sports and exercise
IS - 9
ER -