TY - JOUR
T1 - Endothelial stress by gravitational unloading
T2 - Effects on cell growth and cytoskeletal organization
AU - Carlsson, Sofia I.M.
AU - Bertilaccio, Maria T.S.
AU - Ballabio, Erica
AU - Maier, Jeanette A.M.
N1 - Funding Information:
This work was supported by grants from ASI, ESA and AIRC to JAMM. We thank Dr. Giovanna Mazzoleni, University of Brescia, for her suggestions and advice in the use of the RWV. We are also indebted to Dr. Laura Beguinot for helpful discussions.
PY - 2003/10/21
Y1 - 2003/10/21
N2 - All organisms on Earth have evolved to survive within the pull of gravity. Orbital space flights have clearly demonstrated that the absence or the reduction of gravity profoundly affects eukaryotic organisms, including man. Because (i) endothelial cells are crucial in the maintenance of the functional integrity of the vascular wall, and (ii) cardiovascular deconditioning has been described in astronauts, we evaluated whether microgravity affected endothelial functions. We show that microgravity reversibly stimulated endothelial cell growth. This effect correlated with an overexpression of heat shock protein 70 (hsp70) and a down-regulation of interleukin 1 alpha (IL-1α), a potent inhibitor of endothelial cell growth, also implicated in promoting senescence. In addition, gravitationally unloaded endothelial cells rapidly remodelled their cytoskeleton and, after a few days, markedly down-regulated actin through a transcriptional mechanism. We hypothesize that the reduction in the amounts of actin in response to microgravity represents an adaptative mechanism to avoid the accumulation of redundant actin fibers.
AB - All organisms on Earth have evolved to survive within the pull of gravity. Orbital space flights have clearly demonstrated that the absence or the reduction of gravity profoundly affects eukaryotic organisms, including man. Because (i) endothelial cells are crucial in the maintenance of the functional integrity of the vascular wall, and (ii) cardiovascular deconditioning has been described in astronauts, we evaluated whether microgravity affected endothelial functions. We show that microgravity reversibly stimulated endothelial cell growth. This effect correlated with an overexpression of heat shock protein 70 (hsp70) and a down-regulation of interleukin 1 alpha (IL-1α), a potent inhibitor of endothelial cell growth, also implicated in promoting senescence. In addition, gravitationally unloaded endothelial cells rapidly remodelled their cytoskeleton and, after a few days, markedly down-regulated actin through a transcriptional mechanism. We hypothesize that the reduction in the amounts of actin in response to microgravity represents an adaptative mechanism to avoid the accumulation of redundant actin fibers.
KW - Aging
KW - Cytoskeleton
KW - Endothelial cell
KW - Hsp70
KW - Interleukin 1 alpha
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U2 - 10.1016/j.bbamcr.2003.08.003
DO - 10.1016/j.bbamcr.2003.08.003
M3 - Article
C2 - 14572900
AN - SCOPUS:0142169368
SN - 0167-4889
VL - 1642
SP - 173
EP - 179
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 3
ER -