Abstract
Using the adhesive tumor cell culture system, we studied the effect of epidermal growth factor (EGF) on 16 primary and 7 metastatic fresh human tumors of the nervous system cultured in serum-free and serum-supplemented media at low cell density. In serum-free conditions, EGF significantly enhanced the growth of glial tumor cells. This positive effect was less pronounced for metastases to the brain. No significant enhancement was observed for the other tumor types (primitive neuroectodermal tumors and various tumors of neuroepithelial/mesenchymal origin). The addition of serum obscured this effect of EGF, even at the lowest cell densities. In 7 tumors, the simultaneous addition of platelet-derived growth factor did not enhance the EGF response. Subtypes of brain tumors respond to EGF in vitro under serum-free conditions.
Original language | English (US) |
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Pages (from-to) | 355-359 |
Number of pages | 5 |
Journal | Neurosurgery |
Volume | 23 |
Issue number | 3 |
DOIs | |
State | Published - 1988 |
ASJC Scopus subject areas
- Surgery
- Clinical Neurology