TY - JOUR
T1 - Enhancement of myogenic potential of muscle progenitor cells and muscle healing during pregnancy
AU - Lu, Aiping
AU - Guo, Ping
AU - Pan, Haiying
AU - Tseng, Chieh
AU - Sinha, Krishna
AU - Yang, Fan
AU - Scibetta, Alex
AU - Cui, Yan
AU - Huard, Matthieu
AU - Zhong, Ling
AU - Ravuri, Sudheer
AU - Huard, Johnny
N1 - Publisher Copyright:
© 2021 Federation of American Societies for Experimental Biology
PY - 2021/3
Y1 - 2021/3
N2 - The decline of muscle regenerative potential with age has been attributed to a diminished responsiveness of muscle progenitor cells (MPCs). Heterochronic parabiosis has been used as a model to study the effects of aging on stem cells and their niches. These studies have demonstrated that, by exposing old mice to a young systemic environment, aged progenitor cells can be rejuvenated. One interesting idea is that pregnancy represents a unique biological model of a naturally shared circulatory system between developing and mature organisms. To test this hypothesis, we evaluated the muscle regeneration potential of pregnant mice using a cardiotoxin (CTX) injury mouse model. Our results indicate that the pregnant mice demonstrate accelerated muscle healing compared to nonpregnant control mice following muscle injury based on improved muscle histology, superior muscle regeneration, and a reduction in inflammation and necrosis. Additionally, we found that MPCs isolated from pregnant mice display a significant improvement of myogenic differentiation capacity in vitro and muscle regeneration in vivo when compared to the MPCs from nonpregnant mice. Furthermore, MPCs from nonpregnant mice display enhanced myogenic capacity when cultured in the presence of serum obtained from pregnant mice. Our proteomics data from these studies provides potential therapeutic targets to enhance the myogenic potential of progenitor cells and muscle repair.
AB - The decline of muscle regenerative potential with age has been attributed to a diminished responsiveness of muscle progenitor cells (MPCs). Heterochronic parabiosis has been used as a model to study the effects of aging on stem cells and their niches. These studies have demonstrated that, by exposing old mice to a young systemic environment, aged progenitor cells can be rejuvenated. One interesting idea is that pregnancy represents a unique biological model of a naturally shared circulatory system between developing and mature organisms. To test this hypothesis, we evaluated the muscle regeneration potential of pregnant mice using a cardiotoxin (CTX) injury mouse model. Our results indicate that the pregnant mice demonstrate accelerated muscle healing compared to nonpregnant control mice following muscle injury based on improved muscle histology, superior muscle regeneration, and a reduction in inflammation and necrosis. Additionally, we found that MPCs isolated from pregnant mice display a significant improvement of myogenic differentiation capacity in vitro and muscle regeneration in vivo when compared to the MPCs from nonpregnant mice. Furthermore, MPCs from nonpregnant mice display enhanced myogenic capacity when cultured in the presence of serum obtained from pregnant mice. Our proteomics data from these studies provides potential therapeutic targets to enhance the myogenic potential of progenitor cells and muscle repair.
KW - circulating factors
KW - heterochronic parabiosis
KW - myogenic differentiation
KW - skeletal muscle injury
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U2 - 10.1096/fj.202001914R
DO - 10.1096/fj.202001914R
M3 - Article
C2 - 33565161
AN - SCOPUS:85101335362
SN - 0892-6638
VL - 35
JO - FASEB Journal
JF - FASEB Journal
IS - 3
M1 - e21378
ER -