Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation

Jody Vykoukal; Daynene M. Vykoukal; Susanne Freyberg; Eckhard U. Alt; Peter R.C. Gascoyne

doi:10.1039/b717043b

Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation

Jody Vykoukal, Daynene M. Vykoukal, Susanne Freyberg, Eckhard U. Alt, Peter R.C. Gascoyne

Translational Molecular Pathology

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

We have applied the microfluidic cell separation method of dielectrophoretic field-flow fractionation (DEP-FFF) to the enrichment of a putative stem cell population from an enzyme-digested adipose tissue derived cell suspension. A DEP-FFF separator device was constructed using a novel microfluidic-microelectronic hybrid flex-circuit fabrication approach that is scaleable and anticipates future low-cost volume manufacturing. We report the separation of a nucleated cell fraction from cell debris and the bulk of the erythrocyte population, with the relatively rare (<2% starting concentration) NG2-positive cell population (pericytes and/or putative progenitor cells) being enriched up to 14-fold. This work demonstrates a potential clinical application for DEP-FFF and further establishes the utility of the method for achieving label-free fractionation of cell subpopulations.

Original language	English (US)
Pages (from-to)	1386-1393
Number of pages	8
Journal	Lab on a Chip
Volume	8
Issue number	8
DOIs	https://doi.org/10.1039/b717043b
State	Published - 2008

ASJC Scopus subject areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

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AU - Alt, Eckhard U.

AU - Gascoyne, Peter R.C.

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AB - We have applied the microfluidic cell separation method of dielectrophoretic field-flow fractionation (DEP-FFF) to the enrichment of a putative stem cell population from an enzyme-digested adipose tissue derived cell suspension. A DEP-FFF separator device was constructed using a novel microfluidic-microelectronic hybrid flex-circuit fabrication approach that is scaleable and anticipates future low-cost volume manufacturing. We report the separation of a nucleated cell fraction from cell debris and the bulk of the erythrocyte population, with the relatively rare (<2% starting concentration) NG2-positive cell population (pericytes and/or putative progenitor cells) being enriched up to 14-fold. This work demonstrates a potential clinical application for DEP-FFF and further establishes the utility of the method for achieving label-free fractionation of cell subpopulations.

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Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation

Abstract

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