TY - JOUR
T1 - Establishment and characterization of a human T-cell leukemia Line (LALW-2) in nude mice
AU - White, Les
AU - Meyer, Paul R.
AU - Benedict, William F.
N1 - Funding Information:
I Received August 17, 1983; accepted January 17, 1984. 2 Supported in part by the Concern Foundation. 3 Division of Hematology-Oncology and Clayton Ocular Oncology Center, Childrens Hospital of Los Angeles, 4650 Sunset Blvd., Los Angeles, Calif. 90027. 4 Recipient of a McAlister Research Fellowship at the Childrens Hospital of Los Angeles. 5 Address reprint requests to Dr. White at his present address: Division of Haematology-Oncology, The Prince of Wales Childrens Hospital, High St., Randwick, N.S.W. 2031, Australia. 6 Los Angeles County Hospital, Unversity of Southern California, 1200 North State St., Los Angeles, Calif. 90033. 7 We thank Dr. D. Golde (Department of Medicine, University of California, Los Angeles) f~r analyses of HTLV-related antigens, Dr. S. Rasheed (Department of Pathology, University of Southern California) for analysis of RDP activity, Dr. J. Joncas (Pediatric Research Center, Ste-Justin Hospital, Montreal) for analysis of EBNA, Dr. A. Banerjee for making the chromosome preparations, Mr. Ben Szirth, Mr. Corey Mark, and Mr. Tim Moore for valuable technical assistance, Dr. E. Cifuentes (Guatemala City) and Dr. S. E. Siegel for allowing study of their patient, and Dr. P. P. Dukes for his advice.
PY - 1984/5
Y1 - 1984/5
N2 - Lymphoblasts from the peripheral blood of a 10-year-old boy who was treated for T-cell acute lymphoblastic leukemia were heterotransplanted intraocularly into nude mice. The resultant tumors and their metastases were serially passaged both intraocularly and subcutaneously in the mice. The mouse tumors closely resembled morphologically and cytochemically a human T-cell lymphoma. The primary cells, as well as cells from subsequent in vivo passages, were predominantly of a suppressor T-cell phenotype (OKT8-positive). No viral products were identified. Chromosome analysis revealed a near-diploid karyotype with a translocation between chromosomes 11 and 14. The tumorigenicity, morphology, cytochemistry, immunologic phenotype, and karyotypic pattern of the cells remained constant through six serial in vivo passages over a period of 10 months. This is the first report of direct heterotransplantation and long-term in vivo maintenance of primary human T-cells in immunologically unmanipulated nude mice.
AB - Lymphoblasts from the peripheral blood of a 10-year-old boy who was treated for T-cell acute lymphoblastic leukemia were heterotransplanted intraocularly into nude mice. The resultant tumors and their metastases were serially passaged both intraocularly and subcutaneously in the mice. The mouse tumors closely resembled morphologically and cytochemically a human T-cell lymphoma. The primary cells, as well as cells from subsequent in vivo passages, were predominantly of a suppressor T-cell phenotype (OKT8-positive). No viral products were identified. Chromosome analysis revealed a near-diploid karyotype with a translocation between chromosomes 11 and 14. The tumorigenicity, morphology, cytochemistry, immunologic phenotype, and karyotypic pattern of the cells remained constant through six serial in vivo passages over a period of 10 months. This is the first report of direct heterotransplantation and long-term in vivo maintenance of primary human T-cells in immunologically unmanipulated nude mice.
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U2 - 10.1093/jnci/72.5.1029
DO - 10.1093/jnci/72.5.1029
M3 - Article
C2 - 6609263
AN - SCOPUS:0021276182
SN - 0027-8874
VL - 72
SP - 1029
EP - 1038
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 5
ER -