Estimation of DNA damage and cytotoxicity of anthracycline analogs in human melanoma cells on early and late passages

Elzbieta Anuszewska; Bozena Chłopkiewicz; Beata Gruber; Jadwiga Marczewska; Waldemar Priebe; Henryk Skurzak

Estimation of DNA damage and cytotoxicity of anthracycline analogs in human melanoma cells on early and late passages

Elzbieta Anuszewska, Bozena Chłopkiewicz, Beata Gruber, Jadwiga Marczewska, Waldemar Priebe, Henryk Skurzak

Experimental Therapeutics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Human malignant melanoma is a major problem characterized by both rapid rising incidence and strong chemoresistance. The aim of present experiments was to estimate the effects on DNA of new anthracycline analogs in melanoma cells obtained from various patients, cultured on early (E) and late (L) passages. For determination of cytotoxic effect, MTT assay was used and comet assay was used for the detection of DNA damage. The discrepancy between the intensity of DNA damage processes and IC₅₀ values may indicate that there are some critical loci in the genome, responsible for cell death.

Original language	English (US)
Pages (from-to)	321-324
Number of pages	4
Journal	Acta Poloniae Pharmaceutica - Drug Research
Volume	63
Issue number	4
State	Published - 2006

Keywords

Anthracyclines
Cytotoxicity
DNA damage
Human melanoma

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

Cite this

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abstract = "Human malignant melanoma is a major problem characterized by both rapid rising incidence and strong chemoresistance. The aim of present experiments was to estimate the effects on DNA of new anthracycline analogs in melanoma cells obtained from various patients, cultured on early (E) and late (L) passages. For determination of cytotoxic effect, MTT assay was used and comet assay was used for the detection of DNA damage. The discrepancy between the intensity of DNA damage processes and IC50 values may indicate that there are some critical loci in the genome, responsible for cell death.",

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AU - Anuszewska, Elzbieta

AU - Chłopkiewicz, Bozena

AU - Gruber, Beata

AU - Marczewska, Jadwiga

AU - Priebe, Waldemar

AU - Skurzak, Henryk

PY - 2006

Y1 - 2006

N2 - Human malignant melanoma is a major problem characterized by both rapid rising incidence and strong chemoresistance. The aim of present experiments was to estimate the effects on DNA of new anthracycline analogs in melanoma cells obtained from various patients, cultured on early (E) and late (L) passages. For determination of cytotoxic effect, MTT assay was used and comet assay was used for the detection of DNA damage. The discrepancy between the intensity of DNA damage processes and IC50 values may indicate that there are some critical loci in the genome, responsible for cell death.

AB - Human malignant melanoma is a major problem characterized by both rapid rising incidence and strong chemoresistance. The aim of present experiments was to estimate the effects on DNA of new anthracycline analogs in melanoma cells obtained from various patients, cultured on early (E) and late (L) passages. For determination of cytotoxic effect, MTT assay was used and comet assay was used for the detection of DNA damage. The discrepancy between the intensity of DNA damage processes and IC50 values may indicate that there are some critical loci in the genome, responsible for cell death.

KW - Anthracyclines

KW - Cytotoxicity

KW - DNA damage

KW - Human melanoma

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Estimation of DNA damage and cytotoxicity of anthracycline analogs in human melanoma cells on early and late passages

Abstract

Keywords

ASJC Scopus subject areas

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