Estrogen receptor analysis in chronic lymphocytic leukemia

S. T. Rosen; Z. Maciorowski; F. Wittlin; A. L. Epstein; L. I. Gordon; M. S. Kies; O. Kucuk; H. C. Kwaan; H. Vriesendorp; J. N. Winter

Estrogen receptor analysis in chronic lymphocytic leukemia

S. T. Rosen, Z. Maciorowski, F. Wittlin, A. L. Epstein, L. I. Gordon, M. S. Kies, O. Kucuk, H. C. Kwaan, H. Vriesendorp, J. N. Winter

Thoracic Head & Neck Medical Oncology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Estrogen receptor (ER) determinations were performed on cytosol preparations of Ficoll-Hypaque density separated mononuclear cells from 11 patients with chronic lymphocytic leukemia (CLL). The presence of ER was noted in 8 of 11 specimens (73%), ER ranged from 431 fmole/mg to 4.3 fmole/mg cytosol protein. Two types of receptor subunits were observed at the 8S and 4S region of the sucrose gradient. In addition, 1 of 3 Epstein-Barr virus (EBV) transformed B-lymphoblastoid cell lines from healthy donors had a measurable amount of ER. Patient R.L., who was refractory to standard chemotherapy and radiation and was ER positive, experienced a minor response to Tamoxifen therapy, with subsequent loss of ER. The demonstration of ER in CLL suggests that his malignancy may have a hormone-dependent subpopulation of cells.

Original language	English (US)
Pages (from-to)	996-999
Number of pages	4
Journal	Blood
Volume	62
Issue number	5
State	Published - Dec 1 1983

ASJC Scopus subject areas

Biochemistry
Immunology
Hematology
Cell Biology

Cite this

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title = "Estrogen receptor analysis in chronic lymphocytic leukemia",

abstract = "Estrogen receptor (ER) determinations were performed on cytosol preparations of Ficoll-Hypaque density separated mononuclear cells from 11 patients with chronic lymphocytic leukemia (CLL). The presence of ER was noted in 8 of 11 specimens (73%), ER ranged from 431 fmole/mg to 4.3 fmole/mg cytosol protein. Two types of receptor subunits were observed at the 8S and 4S region of the sucrose gradient. In addition, 1 of 3 Epstein-Barr virus (EBV) transformed B-lymphoblastoid cell lines from healthy donors had a measurable amount of ER. Patient R.L., who was refractory to standard chemotherapy and radiation and was ER positive, experienced a minor response to Tamoxifen therapy, with subsequent loss of ER. The demonstration of ER in CLL suggests that his malignancy may have a hormone-dependent subpopulation of cells.",

author = "Rosen, {S. T.} and Z. Maciorowski and F. Wittlin and Epstein, {A. L.} and Gordon, {L. I.} and Kies, {M. S.} and O. Kucuk and Kwaan, {H. C.} and H. Vriesendorp and Winter, {J. N.}",

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T1 - Estrogen receptor analysis in chronic lymphocytic leukemia

AU - Rosen, S. T.

AU - Maciorowski, Z.

AU - Wittlin, F.

AU - Epstein, A. L.

AU - Gordon, L. I.

AU - Kies, M. S.

AU - Kucuk, O.

AU - Kwaan, H. C.

AU - Vriesendorp, H.

AU - Winter, J. N.

PY - 1983/12/1

Y1 - 1983/12/1

N2 - Estrogen receptor (ER) determinations were performed on cytosol preparations of Ficoll-Hypaque density separated mononuclear cells from 11 patients with chronic lymphocytic leukemia (CLL). The presence of ER was noted in 8 of 11 specimens (73%), ER ranged from 431 fmole/mg to 4.3 fmole/mg cytosol protein. Two types of receptor subunits were observed at the 8S and 4S region of the sucrose gradient. In addition, 1 of 3 Epstein-Barr virus (EBV) transformed B-lymphoblastoid cell lines from healthy donors had a measurable amount of ER. Patient R.L., who was refractory to standard chemotherapy and radiation and was ER positive, experienced a minor response to Tamoxifen therapy, with subsequent loss of ER. The demonstration of ER in CLL suggests that his malignancy may have a hormone-dependent subpopulation of cells.

AB - Estrogen receptor (ER) determinations were performed on cytosol preparations of Ficoll-Hypaque density separated mononuclear cells from 11 patients with chronic lymphocytic leukemia (CLL). The presence of ER was noted in 8 of 11 specimens (73%), ER ranged from 431 fmole/mg to 4.3 fmole/mg cytosol protein. Two types of receptor subunits were observed at the 8S and 4S region of the sucrose gradient. In addition, 1 of 3 Epstein-Barr virus (EBV) transformed B-lymphoblastoid cell lines from healthy donors had a measurable amount of ER. Patient R.L., who was refractory to standard chemotherapy and radiation and was ER positive, experienced a minor response to Tamoxifen therapy, with subsequent loss of ER. The demonstration of ER in CLL suggests that his malignancy may have a hormone-dependent subpopulation of cells.

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Estrogen receptor analysis in chronic lymphocytic leukemia

Abstract

ASJC Scopus subject areas

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