TY - JOUR
T1 - Evaluation of an abbreviated protocol for cytomegalovirus pp65 antigenemia testing
AU - LaSala, P. Rocco
AU - Yarsa, Joe
AU - Nguyen, Dung V.
AU - Han, Xiang Y.
AU - Tarrand, Jeffrey
PY - 2009/4
Y1 - 2009/4
N2 - The cytomegalovirus antigenemia assay remains a useful tool for monitoring reactivation among transplant recipients. In this study, we compared protocols using direct lysis of small-volume, whole blood (WB) samples vs peripheral blood leukocyte (PBL)-enrichedfractions. Of363 evaluable samples, 51 (14.0%) were positive by one or both methods. Sensitivity, specificity, and negative and positive predictive values were similar (76%, 99%, 96%, and 95% vs 71%, 100%, 96%, and 100% for the WB and PBL methods, respectively). Stratification of qualitative results by WBC count revealed comparable detection rates by each method, although the total number of positive results from leukopenic samples was significantly lower than from nonleukopenic samples (P =. 04). Correlation between quantitative results was high, yet the degree of clinical agreement was suboptimal. We conclude that the small-volume, WB lysis method yields results statistically comparable to that of a PBL fractionation method but with fewer technical steps and less complexity.
AB - The cytomegalovirus antigenemia assay remains a useful tool for monitoring reactivation among transplant recipients. In this study, we compared protocols using direct lysis of small-volume, whole blood (WB) samples vs peripheral blood leukocyte (PBL)-enrichedfractions. Of363 evaluable samples, 51 (14.0%) were positive by one or both methods. Sensitivity, specificity, and negative and positive predictive values were similar (76%, 99%, 96%, and 95% vs 71%, 100%, 96%, and 100% for the WB and PBL methods, respectively). Stratification of qualitative results by WBC count revealed comparable detection rates by each method, although the total number of positive results from leukopenic samples was significantly lower than from nonleukopenic samples (P =. 04). Correlation between quantitative results was high, yet the degree of clinical agreement was suboptimal. We conclude that the small-volume, WB lysis method yields results statistically comparable to that of a PBL fractionation method but with fewer technical steps and less complexity.
KW - Antigenemia
KW - Cytomegalovirus
KW - PP65
KW - Peripheral blood leukocytes
KW - Whole blood
UR - http://www.scopus.com/inward/record.url?scp=65349149989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349149989&partnerID=8YFLogxK
U2 - 10.1309/AJCP5C0TRRWYNJXW
DO - 10.1309/AJCP5C0TRRWYNJXW
M3 - Article
C2 - 19289588
AN - SCOPUS:65349149989
SN - 0002-9173
VL - 131
SP - 526
EP - 531
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 4
ER -