Evidence for thyroid hormone receptors in uterine nuclei

Venkat R. Mukku; John L. Kirkland; Marie Hardy; George M. Stancel

doi:10.1016/0026-0495(83)90219-6

Evidence for thyroid hormone receptors in uterine nuclei

Venkat R. Mukku, John L. Kirkland, Marie Hardy, George M. Stancel

Gynecological Oncology & Reproductive Medicine

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Nuclear preparations from the rat uterus contain specific, high affinity binding sites for T₃, with the properties expected of a thyroid hormone receptor. The K_d value for the binding of T₃ to these sites is 9.3 ± 0.1 × 10^-10 M and the maximum number of binding sites is 0.11 ± 0.02 pmoles T₃ bound per mg of DNA. Competition experiments with Triac, D-T₃, T₄, and rT₃ compared to L-T₃ revealed relative affinities of 87%, 22%, 15%, and 1.6% respectively. T₃ binding sites solubilized from uterine nuclear preparations by high salt extraction sediment at 3.6 S and are destroyed largely by trypsin treatment. Estradiol treatment of ovariectomized rats for 3 days did not alter the quantity of T₃ binding sites expressed on the basis of DNA content. These results suggest that uterine nuclei contain a specific T₃ receptor and raise the possibility that this hormone may have direct effects on the uterus.

Original language	English (US)
Pages (from-to)	142-145
Number of pages	4
Journal	Metabolism
Volume	32
Issue number	2
DOIs	https://doi.org/10.1016/0026-0495(83)90219-6
State	Published - Feb 1983

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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title = "Evidence for thyroid hormone receptors in uterine nuclei",

abstract = "Nuclear preparations from the rat uterus contain specific, high affinity binding sites for T3, with the properties expected of a thyroid hormone receptor. The Kd value for the binding of T3 to these sites is 9.3 ± 0.1 × 10-10 M and the maximum number of binding sites is 0.11 ± 0.02 pmoles T3 bound per mg of DNA. Competition experiments with Triac, D-T3, T4, and rT3 compared to L-T3 revealed relative affinities of 87%, 22%, 15%, and 1.6% respectively. T3 binding sites solubilized from uterine nuclear preparations by high salt extraction sediment at 3.6 S and are destroyed largely by trypsin treatment. Estradiol treatment of ovariectomized rats for 3 days did not alter the quantity of T3 binding sites expressed on the basis of DNA content. These results suggest that uterine nuclei contain a specific T3 receptor and raise the possibility that this hormone may have direct effects on the uterus.",

author = "Mukku, {Venkat R.} and Kirkland, {John L.} and Marie Hardy and Stancel, {George M.}",

note = "Funding Information: From the Department of Pharmacology, The University of Texas Medical School at Houston, and Division of Endocrinology, Department of Pediatrics. Baylor College of Medicine, Houston, Texas. Received for publication September 16. 1982. Supported in part by NIH grant HD-08615 and a grant from the Texas Allergy Research Foundation. GMS is the recipient of an NIH Research Career Development Award and JLK is the recipient of an NIH Clinical Investigator Award. Address reprint request to John L. Kirkland. M.D., Division of Endocrinology, Department of Pediatrics, Baylor College of Medicine, 1200 Moursund Ave., Houston, Texas 77030. (c 1983 by Grune & Stratton, Inc. 0026-0495/83/3202-0009$01.00/0",

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AU - Mukku, Venkat R.

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AU - Hardy, Marie

AU - Stancel, George M.

N1 - Funding Information: From the Department of Pharmacology, The University of Texas Medical School at Houston, and Division of Endocrinology, Department of Pediatrics. Baylor College of Medicine, Houston, Texas. Received for publication September 16. 1982. Supported in part by NIH grant HD-08615 and a grant from the Texas Allergy Research Foundation. GMS is the recipient of an NIH Research Career Development Award and JLK is the recipient of an NIH Clinical Investigator Award. Address reprint request to John L. Kirkland. M.D., Division of Endocrinology, Department of Pediatrics, Baylor College of Medicine, 1200 Moursund Ave., Houston, Texas 77030. (c 1983 by Grune & Stratton, Inc. 0026-0495/83/3202-0009$01.00/0

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N2 - Nuclear preparations from the rat uterus contain specific, high affinity binding sites for T3, with the properties expected of a thyroid hormone receptor. The Kd value for the binding of T3 to these sites is 9.3 ± 0.1 × 10-10 M and the maximum number of binding sites is 0.11 ± 0.02 pmoles T3 bound per mg of DNA. Competition experiments with Triac, D-T3, T4, and rT3 compared to L-T3 revealed relative affinities of 87%, 22%, 15%, and 1.6% respectively. T3 binding sites solubilized from uterine nuclear preparations by high salt extraction sediment at 3.6 S and are destroyed largely by trypsin treatment. Estradiol treatment of ovariectomized rats for 3 days did not alter the quantity of T3 binding sites expressed on the basis of DNA content. These results suggest that uterine nuclei contain a specific T3 receptor and raise the possibility that this hormone may have direct effects on the uterus.

AB - Nuclear preparations from the rat uterus contain specific, high affinity binding sites for T3, with the properties expected of a thyroid hormone receptor. The Kd value for the binding of T3 to these sites is 9.3 ± 0.1 × 10-10 M and the maximum number of binding sites is 0.11 ± 0.02 pmoles T3 bound per mg of DNA. Competition experiments with Triac, D-T3, T4, and rT3 compared to L-T3 revealed relative affinities of 87%, 22%, 15%, and 1.6% respectively. T3 binding sites solubilized from uterine nuclear preparations by high salt extraction sediment at 3.6 S and are destroyed largely by trypsin treatment. Estradiol treatment of ovariectomized rats for 3 days did not alter the quantity of T3 binding sites expressed on the basis of DNA content. These results suggest that uterine nuclei contain a specific T3 receptor and raise the possibility that this hormone may have direct effects on the uterus.

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Evidence for thyroid hormone receptors in uterine nuclei

Abstract

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