TY - JOUR
T1 - Evidence of genetic heterogeneity in a BALB/c mouse colony as determined by DNA fingerprinting
AU - Benavides, F.
AU - Cazalla, D.
AU - Pereira, C.
AU - Fontanals, A.
AU - Salaverri, M.
AU - Goldman, A.
AU - Buggiano, V.
AU - Dran, G.
AU - Corley, E.
PY - 1998
Y1 - 1998
N2 - A genetic monitoring of the BALB/c mouse foundation colony in our animal facility was carried out. The techniques of choice were skin grafting, coat colour test, flow cytometric analysis for H2 antigens (loci H2-D and H2-A), electrophoretic analysis of isoenzymes (loci Idh1, Pep3, Es3 and Mod1), PCR-amplified microsatellites (loci Igh-V, Ngfg, Plau, Crp, Igh, D16Mit5, D3Mit49 and D17Mit16) and DNA fingerprinting (multilocus probes 33.6, 33.15 and (CAC)5). No evidence of genetic contamination was found, ruling out the possibility of an outcross with AKR, the other albino strain maintained at the facility. Nevertheless, DNA fingerprint patterns revealed evidence of genetic heterogeneity in four out of nine lines of the nucleus colony, interpreted as minisatellite mutations favoured for a single line system with move than 40 generations of separation from the ancestral pair. These mice are mainly used in cancer and immunological research within the institute.
AB - A genetic monitoring of the BALB/c mouse foundation colony in our animal facility was carried out. The techniques of choice were skin grafting, coat colour test, flow cytometric analysis for H2 antigens (loci H2-D and H2-A), electrophoretic analysis of isoenzymes (loci Idh1, Pep3, Es3 and Mod1), PCR-amplified microsatellites (loci Igh-V, Ngfg, Plau, Crp, Igh, D16Mit5, D3Mit49 and D17Mit16) and DNA fingerprinting (multilocus probes 33.6, 33.15 and (CAC)5). No evidence of genetic contamination was found, ruling out the possibility of an outcross with AKR, the other albino strain maintained at the facility. Nevertheless, DNA fingerprint patterns revealed evidence of genetic heterogeneity in four out of nine lines of the nucleus colony, interpreted as minisatellite mutations favoured for a single line system with move than 40 generations of separation from the ancestral pair. These mice are mainly used in cancer and immunological research within the institute.
KW - DNA fingerprinting
KW - Genetic monitoring
KW - Inbred mice
KW - Strain identification
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U2 - 10.1258/002367798780559400
DO - 10.1258/002367798780559400
M3 - Article
C2 - 9481698
AN - SCOPUS:0031982815
SN - 0023-6772
VL - 32
SP - 80
EP - 85
JO - Laboratory Animals
JF - Laboratory Animals
IS - 1
ER -