TY - JOUR
T1 - Experimental strategies of the application of RNAi technique in mammalian cells
AU - Zhang, Ding Xiao
AU - Pan, Bin
AU - Liu, Bang
AU - Li, Kui
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 2005
Y1 - 2005
N2 - As a novel and effective tool for gene function analysis, RNA interference (RNAi) technique has been developed rapidly and made great progress in mammalian cell studies. The mechanisms and experimental operation of RNAi in mammalian cells have become research hotspots, and RNAi mechanisms were supposed to be different from those in plant. In this paper, a review of RNAi mechanisms, the latest research progress and the experimental strategies of RNAi in mammalian cells were presented, including siRNA sequence design, siRNA delivery approaches and RNAi effect detection, so as to offer valuable references for RNAi studies.
AB - As a novel and effective tool for gene function analysis, RNA interference (RNAi) technique has been developed rapidly and made great progress in mammalian cell studies. The mechanisms and experimental operation of RNAi in mammalian cells have become research hotspots, and RNAi mechanisms were supposed to be different from those in plant. In this paper, a review of RNAi mechanisms, the latest research progress and the experimental strategies of RNAi in mammalian cells were presented, including siRNA sequence design, siRNA delivery approaches and RNAi effect detection, so as to offer valuable references for RNAi studies.
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M3 - Review article
C2 - 16257920
AN - SCOPUS:58149237203
SN - 0253-9772
VL - 27
SP - 839
EP - 844
JO - Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji
JF - Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji
IS - 5
ER -