Exploring the concordance of aspergillus fumigatus pathogenicity in mice and Toll-deficient flies

Georgios Chamilos; Elaine M. Bignell; Markus Schrettl; Russell E. Lewis; Konstantinos Leventakos; Gregory S. May; Hubertus Haas; Dimitrios P. Kontoyiannis

doi:10.3109/13693780903225813

Exploring the concordance of aspergillus fumigatus pathogenicity in mice and Toll-deficient flies

Georgios Chamilos, Elaine M. Bignell, Markus Schrettl, Russell E. Lewis, Konstantinos Leventakos, Gregory S. May, Hubertus Haas, Dimitrios P. Kontoyiannis

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27 Scopus citations

Abstract

The pathogenicity of six mutants of Aspergillus fumigatus that had been previously characterized in mice was assessed in Toll-deficient Drosophila melanogaster flies. Four out of six mutants of A. fumigatus, which displayed attenuated virulence in mice due to defects in siderophore biosynthesis (ΔsidA, ΔsidD), PABA metabolism (H515), and starvation stress response (ΔcpcA), also had attenuated virulence in the fly model. In addition, similarly to previous findings in the mouse model, ΔsidG mutant that is defective in extracellular siderophore biosynthesis retained full virulence in Toll-deficient flies. Overall, our studies reveal a high level of concordance between fly and murine models of invasive aspergillosis.

Original language	English (US)
Pages (from-to)	506-510
Number of pages	5
Journal	Medical mycology
Volume	48
Issue number	3
DOIs	https://doi.org/10.3109/13693780903225813
State	Published - 2010

Keywords

Aspergillus
Drosophila melanogaster
Invertebrates
Toll receptor
Virulence

ASJC Scopus subject areas

Infectious Diseases

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10.3109/13693780903225813

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title = "Exploring the concordance of aspergillus fumigatus pathogenicity in mice and Toll-deficient flies",

abstract = "The pathogenicity of six mutants of Aspergillus fumigatus that had been previously characterized in mice was assessed in Toll-deficient Drosophila melanogaster flies. Four out of six mutants of A. fumigatus, which displayed attenuated virulence in mice due to defects in siderophore biosynthesis (ΔsidA, ΔsidD), PABA metabolism (H515), and starvation stress response (ΔcpcA), also had attenuated virulence in the fly model. In addition, similarly to previous findings in the mouse model, ΔsidG mutant that is defective in extracellular siderophore biosynthesis retained full virulence in Toll-deficient flies. Overall, our studies reveal a high level of concordance between fly and murine models of invasive aspergillosis.",

keywords = "Aspergillus, Drosophila melanogaster, Invertebrates, Toll receptor, Virulence",

author = "Georgios Chamilos and Bignell, {Elaine M.} and Markus Schrettl and Lewis, {Russell E.} and Konstantinos Leventakos and May, {Gregory S.} and Hubertus Haas and Kontoyiannis, {Dimitrios P.}",

note = "Funding Information: Financial support was provided by the M. D. Anderson Cancer Center (an institutional research grant and the M. D. Anderson Faculty E. N. Cobb Scholar Award Research Endowment to D.P.K.) and Austrian Science Foundation [FWF P-18606-B11 to H.H.] Declaration of interest: The authors have no conflicts of interest to declare. The authors alone are responsible for the content and writing of this paper.",

year = "2010",

doi = "10.3109/13693780903225813",

language = "English (US)",

volume = "48",

pages = "506--510",

journal = "Medical mycology",

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number = "3",

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TY - JOUR

T1 - Exploring the concordance of aspergillus fumigatus pathogenicity in mice and Toll-deficient flies

AU - Chamilos, Georgios

AU - Bignell, Elaine M.

AU - Schrettl, Markus

AU - Lewis, Russell E.

AU - Leventakos, Konstantinos

AU - May, Gregory S.

AU - Haas, Hubertus

AU - Kontoyiannis, Dimitrios P.

N1 - Funding Information: Financial support was provided by the M. D. Anderson Cancer Center (an institutional research grant and the M. D. Anderson Faculty E. N. Cobb Scholar Award Research Endowment to D.P.K.) and Austrian Science Foundation [FWF P-18606-B11 to H.H.] Declaration of interest: The authors have no conflicts of interest to declare. The authors alone are responsible for the content and writing of this paper.

PY - 2010

Y1 - 2010

N2 - The pathogenicity of six mutants of Aspergillus fumigatus that had been previously characterized in mice was assessed in Toll-deficient Drosophila melanogaster flies. Four out of six mutants of A. fumigatus, which displayed attenuated virulence in mice due to defects in siderophore biosynthesis (ΔsidA, ΔsidD), PABA metabolism (H515), and starvation stress response (ΔcpcA), also had attenuated virulence in the fly model. In addition, similarly to previous findings in the mouse model, ΔsidG mutant that is defective in extracellular siderophore biosynthesis retained full virulence in Toll-deficient flies. Overall, our studies reveal a high level of concordance between fly and murine models of invasive aspergillosis.

AB - The pathogenicity of six mutants of Aspergillus fumigatus that had been previously characterized in mice was assessed in Toll-deficient Drosophila melanogaster flies. Four out of six mutants of A. fumigatus, which displayed attenuated virulence in mice due to defects in siderophore biosynthesis (ΔsidA, ΔsidD), PABA metabolism (H515), and starvation stress response (ΔcpcA), also had attenuated virulence in the fly model. In addition, similarly to previous findings in the mouse model, ΔsidG mutant that is defective in extracellular siderophore biosynthesis retained full virulence in Toll-deficient flies. Overall, our studies reveal a high level of concordance between fly and murine models of invasive aspergillosis.

KW - Aspergillus

KW - Drosophila melanogaster

KW - Invertebrates

KW - Toll receptor

KW - Virulence

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Exploring the concordance of aspergillus fumigatus pathogenicity in mice and Toll-deficient flies

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Keywords

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