TY - JOUR
T1 - [Expression and exon 3 mutation of beta-catenin in human hepatocellular carcinoma].
AU - Jiao, Yang
AU - Ban, Ke Chen
AU - Cao, J.
AU - Yue, Hai Ying
AU - Luo, Yuan
AU - Su, Jian Jia
PY - 2007/10
Y1 - 2007/10
N2 - South Guangxi is an area with high incidence of hepatocellular carcinoma (HCC), and with severe contamination of dietary aflatoxin B1 (AFB1). The activation of beta-Catenin is involved in many cancers. AFB1 may play a key role in hepatocarcinogenesis. This study was to explore the expression and mutation of beta-Catenin in HCC patients from the area with high exposure level of AFB1. The expression of beta-Catenin in 52 specimens of HCC and para-HCC tissues, and 18 specimens of non-cancerous liver tissues from South Guangxi were detected by direct sequencing, reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot. No mutation in exon 3 of beta-Catenin gene was found in HCC tissues. The mRNA level of beta-Catenin was significantly higher in HCC tissues than in para-HCC tissues and non-cancerous tissues (0.42+/-0.24 vs. 0.20+/-0.16 and 0.23+/-0.12, P<0.01). The positive rate of beta-Catenin was significantly higher in HCC tissues than in para-HCC tissues (55.8% vs. 36.5%, P<0.05). The expression of beta-Catenin mRNA showed no significant correlation to clinicopathologic parameters of HCC (all P>0.05), while the expression of beta-Catenin protein was significantly correlated to metastasis, relapse, portal vein embolus, and clinical stage (all P<0.05). Beta-catenin is overexpressed in HCC, but its overexpression has no correlation to gene mutation at GSK-3beta phosphorylation sites in exon 3.
AB - South Guangxi is an area with high incidence of hepatocellular carcinoma (HCC), and with severe contamination of dietary aflatoxin B1 (AFB1). The activation of beta-Catenin is involved in many cancers. AFB1 may play a key role in hepatocarcinogenesis. This study was to explore the expression and mutation of beta-Catenin in HCC patients from the area with high exposure level of AFB1. The expression of beta-Catenin in 52 specimens of HCC and para-HCC tissues, and 18 specimens of non-cancerous liver tissues from South Guangxi were detected by direct sequencing, reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot. No mutation in exon 3 of beta-Catenin gene was found in HCC tissues. The mRNA level of beta-Catenin was significantly higher in HCC tissues than in para-HCC tissues and non-cancerous tissues (0.42+/-0.24 vs. 0.20+/-0.16 and 0.23+/-0.12, P<0.01). The positive rate of beta-Catenin was significantly higher in HCC tissues than in para-HCC tissues (55.8% vs. 36.5%, P<0.05). The expression of beta-Catenin mRNA showed no significant correlation to clinicopathologic parameters of HCC (all P>0.05), while the expression of beta-Catenin protein was significantly correlated to metastasis, relapse, portal vein embolus, and clinical stage (all P<0.05). Beta-catenin is overexpressed in HCC, but its overexpression has no correlation to gene mutation at GSK-3beta phosphorylation sites in exon 3.
UR - http://www.scopus.com/inward/record.url?scp=67749085446&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67749085446&partnerID=8YFLogxK
M3 - Article
C2 - 17927878
AN - SCOPUS:67749085446
SN - 1000-467X
VL - 26
SP - 1085
EP - 1089
JO - Ai zheng = Aizheng = Chinese journal of cancer
JF - Ai zheng = Aizheng = Chinese journal of cancer
IS - 10
ER -