Expression and Purification of Recombinant Proteins Using the Baculovirus System

Cheryl Isaac Murphy; Helen Piwnica-Worms; Stefan Grünwald; William G. Romanow; Nicole Francis; Hua Ying Fan; Sharon Marr

doi:10.1002/cpmb.61

Expression and Purification of Recombinant Proteins Using the Baculovirus System

Cheryl Isaac Murphy, Helen Piwnica-Worms, Stefan Grünwald, William G. Romanow, Nicole Francis, Hua Ying Fan, Sharon Marr

Research output: Contribution to journal › Article › peer-review

Abstract

This article describes how to analyze protein expression in cells infected with recombinant baculovirus on a small scale for optimizing protein production, how to maximize and scale up recombinant protein production, and how to purify recombinant proteins.

Original language	English (US)
Article number	e61
Journal	Current protocols in molecular biology
Volume	123
Issue number	1
DOIs	https://doi.org/10.1002/cpmb.61
State	Published - Jul 2018
Externally published	Yes

Keywords

baculovirus
expression
purification
recombinant protein

ASJC Scopus subject areas

Molecular Biology

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10.1002/cpmb.61

Cite this

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title = "Expression and Purification of Recombinant Proteins Using the Baculovirus System",

abstract = "This article describes how to analyze protein expression in cells infected with recombinant baculovirus on a small scale for optimizing protein production, how to maximize and scale up recombinant protein production, and how to purify recombinant proteins.",

keywords = "baculovirus, expression, purification, recombinant protein",

author = "Murphy, {Cheryl Isaac} and Helen Piwnica-Worms and Stefan Gr{\"u}nwald and Romanow, {William G.} and Nicole Francis and Fan, {Hua Ying} and Sharon Marr",

note = "Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons, Inc.",

year = "2018",

month = jul,

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language = "English (US)",

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AU - Murphy, Cheryl Isaac

AU - Piwnica-Worms, Helen

AU - Grünwald, Stefan

AU - Romanow, William G.

AU - Francis, Nicole

AU - Fan, Hua Ying

AU - Marr, Sharon

PY - 2018/7

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KW - expression

KW - purification

KW - recombinant protein

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Expression and Purification of Recombinant Proteins Using the Baculovirus System

Abstract

Keywords

ASJC Scopus subject areas

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