Ekspressiia v kletkakh Escherichia coli nukleotidnoǐ posledovatel'nosti DNK, kodiruiushcheǐ lipotropnyǐ gormon byka.

A. B. Beklemishev; S. I. Golovin; A. A. Il'ichev; L. V. Mamaev; V. N. Krasnykh

Ekspressiia v kletkakh Escherichia coli nukleotidnoǐ posledovatel'nosti DNK, kodiruiushcheǐ lipotropnyǐ gormon byka.

Translated title of the contribution: Expression in Escherichia coli cells of the nucleotide sequence of DNA coding for the bovine lipotropic hormone

A. B. Beklemishev, S. I. Golovin, A. A. Il'ichev, L. V. Mamaev, V. N. Krasnykh

Cancer Systems Imaging

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A cDNA fragment of bovine proopiomelanocortin coding for beta-lipotropic hormone was joined with a promoter and ribosome binding site of B. amyloliquefaciens and cloned in E. coli in pBR 327 plasmid. The level of beta-lipotropin synthesis in bacterial cells transformed by the obtained plasmid was estimated immunochemically. The level of beta-lipotropin production was shown to be 5 mg per liter of bacterial culture.

Translated title of the contribution	Expression in Escherichia coli cells of the nucleotide sequence of DNA coding for the bovine lipotropic hormone
Original language	Russian
Pages (from-to)	19-23
Number of pages	5
Journal	Molekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number	10
State	Published - Oct 1987

ASJC Scopus subject areas

General Medicine

Cite this

@article{679e534056aa49829072dd33622df784,

title = "Ekspressiia v kletkakh Escherichia coli nukleotidnoǐ posledovatel'nosti DNK, kodiruiushcheǐ lipotropnyǐ gormon byka.",

abstract = "A cDNA fragment of bovine proopiomelanocortin coding for beta-lipotropic hormone was joined with a promoter and ribosome binding site of B. amyloliquefaciens and cloned in E. coli in pBR 327 plasmid. The level of beta-lipotropin synthesis in bacterial cells transformed by the obtained plasmid was estimated immunochemically. The level of beta-lipotropin production was shown to be 5 mg per liter of bacterial culture.",

author = "Beklemishev, {A. B.} and Golovin, {S. I.} and Il'ichev, {A. A.} and Mamaev, {L. V.} and Krasnykh, {V. N.}",

note = "Copyright: Medline is the source for the citation and abstract of this record.",

year = "1987",

month = oct,

language = "Russian",

pages = "19--23",

journal = "Molekuliarnaia genetika, mikrobiologiia i virusologiia",

issn = "0208-0613",

publisher = "Meditsina Publishers",

number = "10",

}

TY - JOUR

T1 - Ekspressiia v kletkakh Escherichia coli nukleotidnoǐ posledovatel'nosti DNK, kodiruiushcheǐ lipotropnyǐ gormon byka.

AU - Beklemishev, A. B.

AU - Golovin, S. I.

AU - Il'ichev, A. A.

AU - Mamaev, L. V.

AU - Krasnykh, V. N.

N1 - Copyright: Medline is the source for the citation and abstract of this record.

PY - 1987/10

Y1 - 1987/10

N2 - A cDNA fragment of bovine proopiomelanocortin coding for beta-lipotropic hormone was joined with a promoter and ribosome binding site of B. amyloliquefaciens and cloned in E. coli in pBR 327 plasmid. The level of beta-lipotropin synthesis in bacterial cells transformed by the obtained plasmid was estimated immunochemically. The level of beta-lipotropin production was shown to be 5 mg per liter of bacterial culture.

AB - A cDNA fragment of bovine proopiomelanocortin coding for beta-lipotropic hormone was joined with a promoter and ribosome binding site of B. amyloliquefaciens and cloned in E. coli in pBR 327 plasmid. The level of beta-lipotropin synthesis in bacterial cells transformed by the obtained plasmid was estimated immunochemically. The level of beta-lipotropin production was shown to be 5 mg per liter of bacterial culture.

UR - http://www.scopus.com/inward/record.url?scp=0023431216&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023431216&partnerID=8YFLogxK

M3 - Article

C2 - 3323897

AN - SCOPUS:0023431216

SN - 0208-0613

SP - 19

EP - 23

JO - Molekuliarnaia genetika, mikrobiologiia i virusologiia

JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia

IS - 10

ER -

Ekspressiia v kletkakh Escherichia coli nukleotidnoǐ posledovatel'nosti DNK, kodiruiushcheǐ lipotropnyǐ gormon byka.

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this