Abstract
12-Lipoxygenase (12-LOX), through its metabolite 12(S)- hydroxyeicosatetraenoic acid [12(S)-HETE], has been demonstrated to play a pivotal role in experimental melanoma invasion and metastasis, and 12-LOX expression may be important in early human melanoma carcinogenesis. We have studied the differences in 12-LOX protein expression during the progression of melanoma from human melanocytic cells to benign and dysplastic naevi to malignant metastatic disease. 12-LOX expression was determined in normal human skin melanocytes and in melanocytes found in compound naevi, dysplastic naevi and melanomas using a platelet-type 12-LOX antibody with a diaminobenzidine immunoperoxidase system detection system and was quantified using the analysis software NIH Image 1.62. Mean cellular pixel densities for 12-LOX staining (n = 50 cells/histological type) were unchanged in compound naevi (P = 0.14) and were increased in dysplastic naevi and melanomas compared with normal skin melanocytes (P = 0.03 and P = 0.01, respectively). Similarly, melanomas had higher levels of expression compared with dysplastic naevi (P = 0.03). 12-LOX expression was significantly different between compound naevus and dysplastic naevus melanocytes (P = 0.01). These data suggest that 12-LOX may be an important novel marker for cancer progression within the melanoma system, and therefore could be a useful biomarker and therapeutic target for melanoma chemoprevention.
Original language | English (US) |
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Pages (from-to) | 429-434 |
Number of pages | 6 |
Journal | Melanoma research |
Volume | 12 |
Issue number | 5 |
DOIs | |
State | Published - Sep 2002 |
Keywords
- Dysplastic naevus
- Lipoxygenase
- Melanoma
ASJC Scopus subject areas
- Oncology
- Dermatology
- Cancer Research