TY - JOUR
T1 - Expression of aerobactin genes by Shigella flexneri during extracellular and intracellular growth
AU - Headley, V.
AU - Hong, M.
AU - Galko, M.
AU - Payne, S. M.
PY - 1997
Y1 - 1997
N2 - The expression of the Shigella flexneri chromosomal aerobactin genes during growth of the bacterium within tissue culture cells was assayed. During intracellular growth, aerobactin promoter activity was repressed relative to the level observed in bacteria grown extracellularly, even when the bacteria had been starved for iron prior to infection. Similarly, the level of one of the proteins encoded by this operon, the aerobactin outer membrane receptor, Iut, was reduced in the intracellular environment. These studies indicate that the aerobactin system is not highly expressed by bacteria within host cells, suggesting that siderophore-independent iron acquisition systems can provide essential iron during intracellular multiplication.
AB - The expression of the Shigella flexneri chromosomal aerobactin genes during growth of the bacterium within tissue culture cells was assayed. During intracellular growth, aerobactin promoter activity was repressed relative to the level observed in bacteria grown extracellularly, even when the bacteria had been starved for iron prior to infection. Similarly, the level of one of the proteins encoded by this operon, the aerobactin outer membrane receptor, Iut, was reduced in the intracellular environment. These studies indicate that the aerobactin system is not highly expressed by bacteria within host cells, suggesting that siderophore-independent iron acquisition systems can provide essential iron during intracellular multiplication.
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U2 - 10.1128/iai.65.2.818-821.1997
DO - 10.1128/iai.65.2.818-821.1997
M3 - Article
C2 - 9009350
AN - SCOPUS:0031029140
SN - 0019-9567
VL - 65
SP - 818
EP - 821
JO - Infection and Immunity
JF - Infection and Immunity
IS - 2
ER -