@article{482507d6d28643dda14fcd4856c48a6f,
title = "Expression of p16, Rb, and cyclin D1 gene products in oral and laryngeal squamous carcinoma: Biological and clinical implications",
abstract = "Cyclin D1, p16, and Rb genes play a critical role in the regulation of the G1-S transition of the cell cycle and are frequently altered in several neoplastic entities. Analysis of the protein products of these genes by molecular and immunohistochemical methods provides information on their functional status and allows for the phenotypic evaluation of tumor cells. We performed Western blotting and immunohistochemical analysis on tissues from 35 primary oral and laryngeal squamous carcinoma specimens with previous molecular analysis of the p16 gene and correlated the results with relevant clinicopathologic factors. Our study shows significant concordance between Western blotting and immunostaining results for cyclin D1 (P = .01), p16 proteins (P = .01), and Rb (P = .04). Heterogeneous staining of tumor cells and the positivity of non-neoplastic host elements for Rb by immunohistochemistry contributed to the discrepancy noted in some tumors by Western blotting. Significant reciprocal relationship between p16 and Rb proteins was observed (P < .001); in most tumors, absence of p16 (89%) and detectable Rb (94%) proteins were found. Two tumors had negative cyclin D1 expression, and one third overexpressed this protein. There was a lack of correlation between cyclin D1 overexpression and the clinicopathologic factors studied. Our results indicate that the absence of p16 in most of these tumors may constitute an early tumorigenic event and that the loss of the Rb function plays a minor role in HNSC.",
keywords = "Cell cycle, Head and neck squamous carcinoma, Immunohistochemistry, Tumor suppressor gene, Western blotting",
author = "El-Naggar, {Adel K.} and Syeling Lai and Clayman, {Gary L.} and Zhou, {Jain Hua} and Tucker, {Susan A.} and Jeffrey Myers and Luna, {Mario A.} and Benedict, {William F.}",
note = "Funding Information: From the Departments of Pathology, Biomathematics, Head and Neck Surgery, and Genitourinary Medical Ontology, The University of Texas M.D. Anderson Cancer Center, Houston, TX. Accepted for publication April 20, 1999. Supported by grant 1P50DE11906-01 from the National Institute of DentaI Health to AEN and CA54672 from the NCI to W.B. Address correspondence and reprint requests to Adel K. E1-Naggar, MD, PhD, Department of Pathology, The University of Texas, M.D. Anderson Cancer Center, 1515 Holcombe Blvd, Box 85, Houston, TX 77030. Copyright {\textcopyright} 1999 by W.B. Saunders Company 0046-8177/99/3009-0003510.00/0 ancy noted in some tumors by Western blotting. Significant reciprocal relationship between pl6 and Rb proteins was observed (P < .001); in most tumors, absence of pl6 (89%) and detectable Rb (94%) proteins were found. Two tumors had negative cyclin D1 expression, and one third overexpressed this protein. There was a lack of correlation between cyclin D1 overexpression and the clinicopathologic factors studied. Our results indicate that the absence of p16 in most of these tumors may constitute an early tumorigenic event and that the loss of the Rb function plays a minor role in HNSC. HUM PATROL 30:1013-1018. Copyright {\textcopyright} 1999 by W.B. Saunders Company Key words: head and neck squamons carcinoma, cell cycle, tumor suppressor gene, immunohistochemistry, Western blotting. Abbreviations: HNSC, head and neck squamous carcinoma; IHC, immunohistochemistry; WB~ Western blotting.",
year = "1999",
doi = "10.1016/S0046-8177(99)90217-4",
language = "English (US)",
volume = "30",
pages = "1013--1018",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "9",
}