Abstract
Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 112-115 |
| Number of pages | 4 |
| Journal | Sheng wu gong cheng xue bao = Chinese journal of biotechnology |
| Volume | 19 |
| Issue number | 1 |
| State | Published - Jan 2003 |
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology