TY - JOUR
T1 - Expression of transforming growth factor-β2 in malignant melanoma correlates with the depth of tumor invasion
T2 - Implications for tumor progression
AU - Reed, Jon A.
AU - McNutt, N. Scott
AU - Prieto, Victor G.
AU - Albino, Anthony P.
PY - 1994/7
Y1 - 1994/7
N2 - Transforming growth factor-β2 (TGF-β2) is a potent regulator of proliferation and differentiation of both normal and malignant cells. In addition, TGF-β2 can exert a variety of immunosuppressive effects, suggesting that the production of this molecule contributes to impaired immunological surveillance of tumor development. In vitro, TGF-β2 expression has been demonstrated in cell lines derived from metastatic malignant melanomas, but not in those derived from normal melanocytes. We sought to evaluate a potential role of TGF-β2 in the initiation or progression of malignant melanoma in vivo. We examined by nucleic acid in situ hybridization the expression of TGF-β2 mRNA transcripts in 124 melanocytic lesions including metastatic and primary invasive melanomas, melanomas in situ, nevi with architectural disorder and cytologic atypia, ordinary benign melanocytic nevi, and Spitz nevi. All metastatic melanomas and a majority (94%) of primary melanomas invasive to Clark's level III, IV, or V expressed TGF-β2 mRNA. A minority (41%) of Clark's level II primary invasive melanomas expressed this factor. All definitive melanomas in situ and nevi were negative. The results suggest that TGF-β2 expression in malignant melanoma may be a critical event in the development of deep invasion and metastases in malignant melanoma.
AB - Transforming growth factor-β2 (TGF-β2) is a potent regulator of proliferation and differentiation of both normal and malignant cells. In addition, TGF-β2 can exert a variety of immunosuppressive effects, suggesting that the production of this molecule contributes to impaired immunological surveillance of tumor development. In vitro, TGF-β2 expression has been demonstrated in cell lines derived from metastatic malignant melanomas, but not in those derived from normal melanocytes. We sought to evaluate a potential role of TGF-β2 in the initiation or progression of malignant melanoma in vivo. We examined by nucleic acid in situ hybridization the expression of TGF-β2 mRNA transcripts in 124 melanocytic lesions including metastatic and primary invasive melanomas, melanomas in situ, nevi with architectural disorder and cytologic atypia, ordinary benign melanocytic nevi, and Spitz nevi. All metastatic melanomas and a majority (94%) of primary melanomas invasive to Clark's level III, IV, or V expressed TGF-β2 mRNA. A minority (41%) of Clark's level II primary invasive melanomas expressed this factor. All definitive melanomas in situ and nevi were negative. The results suggest that TGF-β2 expression in malignant melanoma may be a critical event in the development of deep invasion and metastases in malignant melanoma.
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M3 - Article
C2 - 8030760
AN - SCOPUS:0028341206
SN - 0002-9440
VL - 145
SP - 97
EP - 104
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 1
ER -