TY - JOUR
T1 - Fiber optic probe for polarized reflectance spectroscopy in vivo
T2 - Design and performance
AU - Myakov, Alexey
AU - Nieman, Linda
AU - Wicky, Lorenz
AU - Utzinger, Urs
AU - Richards-Kortum, Rebecca
AU - Sokolov, Konstantin
N1 - Funding Information:
The authors acknowledge the contribution of Dafna Lotan for preparation of specimens. They thank Dr. Reuben Lotan of the University of Texas M. D. Anderson Cancer Center for pro- viding specimen cultures of cells. Financial support from the Whitaker Foundation is gratefully acknowledged.
PY - 2002/7
Y1 - 2002/7
N2 - We present the design and construction of a fiber optic probe for elastic light scattering spectroscopy in vivo with polarized excitation and polarization sensitive detection. The performance of the fiber probe is evaluated using a suspension of polystyrene spheres placed atop a diffusely scattering substrate, and it demonstrates that the size-dependent characteristics of the scatterers can be extracted in the presence of a highly diffusely scattering background using a linear combination of forward and backward Mie scattering components of the scatterers. Subsequently, Mie theory calculations are performed over a broad range of diagnostically relevant parameters of nuclei-mean diameter, size distribution, and relative refractive index-to understand how the polarized reflectance measurements with the fiber probe can be used to extract morphological information about epithelial tissue. Finally, the feasibility of in vivo measurements with the fiber optic based polarization sensitive light scattering spectroscopy is demonstrated.
AB - We present the design and construction of a fiber optic probe for elastic light scattering spectroscopy in vivo with polarized excitation and polarization sensitive detection. The performance of the fiber probe is evaluated using a suspension of polystyrene spheres placed atop a diffusely scattering substrate, and it demonstrates that the size-dependent characteristics of the scatterers can be extracted in the presence of a highly diffusely scattering background using a linear combination of forward and backward Mie scattering components of the scatterers. Subsequently, Mie theory calculations are performed over a broad range of diagnostically relevant parameters of nuclei-mean diameter, size distribution, and relative refractive index-to understand how the polarized reflectance measurements with the fiber probe can be used to extract morphological information about epithelial tissue. Finally, the feasibility of in vivo measurements with the fiber optic based polarization sensitive light scattering spectroscopy is demonstrated.
KW - Cancer diagnosis
KW - Elastic light scattering
KW - Polarized light
KW - Reflectance spectroscopy
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U2 - 10.1117/1.1483314
DO - 10.1117/1.1483314
M3 - Article
C2 - 12175288
AN - SCOPUS:0036629492
SN - 1083-3668
VL - 7
SP - 388
EP - 397
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 3
ER -