TY - JOUR
T1 - Fibrin overlay methods for the detection of single transformed cells and colonies of transformed cells
AU - Jones, Peter
AU - Benedict, William
AU - Strickland, Sidney
AU - Reich, E.
N1 - Funding Information:
We are grateful to Samuel Barst for expert technical assistance. W. 6. is a recipient of a Career Development Award from the National institute of Health, and S. S. is a fellow of the Jane Coffin Childs Memorial Fund for Medical Research. Supported by grants and a contract from the Public Health Service, National Cancer Institute, and the American Cancer Society.
PY - 1975/7
Y1 - 1975/7
N2 - Fibrin overlay methods are described which can detect the plasminogen activator produced by single transformed cells or small colonies of transformed cells. These methods were applied to malignant cells derived from humans, mice, hamsters, rats, and chicks. The lysis observed was plasminogen dependent. Transformation of chicken cells by Rous sarcoma virus was detected 4 days after infection. The number of lysis zones produced was proportional to the virus inoculum and was identical to the number of morphologically determined foci. These methods may also have application in model systems for scoring transformation by chemicals. Transformed mouse and chicken cells were detected at the single cell level and the number of lysis zones produced was dependent on the number of cells present, the time of incubation, and the concentration of plasminogen.
AB - Fibrin overlay methods are described which can detect the plasminogen activator produced by single transformed cells or small colonies of transformed cells. These methods were applied to malignant cells derived from humans, mice, hamsters, rats, and chicks. The lysis observed was plasminogen dependent. Transformation of chicken cells by Rous sarcoma virus was detected 4 days after infection. The number of lysis zones produced was proportional to the virus inoculum and was identical to the number of morphologically determined foci. These methods may also have application in model systems for scoring transformation by chemicals. Transformed mouse and chicken cells were detected at the single cell level and the number of lysis zones produced was dependent on the number of cells present, the time of incubation, and the concentration of plasminogen.
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U2 - 10.1016/0092-8674(75)90108-7
DO - 10.1016/0092-8674(75)90108-7
M3 - Article
C2 - 167984
AN - SCOPUS:0016715222
SN - 0092-8674
VL - 5
SP - 323
EP - 329
JO - Cell
JF - Cell
IS - 3
ER -