Abstract
Neoplastic transformation of glial cells alters inclusion of the α exon in human fibroblast growth factor receptor-1 (FGFR-1) mRNA transcripts. Although normal cells predominantly include the α exon, this exon is excluded in most glioblastoma cell transcripts, creating a high-affinity receptor form. In this study, we identified polypyrimidine tract-binding protein (PTB) as a regulator of FGFR-1 splicing. PTB interacted in a sequence-specific manner with the ISS-1 regulatory element in the intron upstream of the α exon. PTB expression was also strongly increased in seven malignant glioblastoma multiforme tumors relative to adjacent normal tissue, but not in a low-grade astrocytoma. These results suggest that increased expression of PTB may contribute to glial cell malignancy.
Original language | English (US) |
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Pages (from-to) | 1221-1224 |
Number of pages | 4 |
Journal | Cancer Research |
Volume | 60 |
Issue number | 5 |
State | Published - Mar 1 2000 |
ASJC Scopus subject areas
- Oncology
- Cancer Research