Abstract
Sixteen fine needle aspiration (FNA) biopsies of lymphoblastic lymphoma (LBL) that were used to either initially diagnose disease (12) or document relapse (4) were reviewed. Cellular aspirates (2 x 107 cells) were readily obtained for immunologic, DNA/RNA flow cytometric and immunoglobulin and/or T-cell receptor gene rearrangement studies. Cytologically, aspirates were characterized by intermediate-sized cells (9.5-18.5 μm) with fine nuclear chromatin, small, inconspicuous nucleoli, irregular nuclear contours and scant basophilic cytoplasm. Frequent mitotic figures were seen (1-14 figures per 1,000 cells). Fourteen cases demonstrated a T-cell phenotype with considerable phenotypic variability. One case demonstrated a precursor B- cell phenotype, and another demonstrated biphenotypic expression with both T- cell and myeloid differentiation. Eleven of 14 cases (79%) were positive for terminal deoxynucleotidyl transferase. Thirteen of 15 cases (87%) manifested diploid DNA content by flow cytometric analysis and were characterized by intermediate proliferative activity (S + G2M 12.7 ± 8.7% SD) and intermediate mean RNA index (1.3 ± .5 SD). Tβ gene rearrangements were demonstrated in four of five phenotypic T-cell LBL cases analyzed, with concomitant J(H) gene rearrangements observed in three cases, confirming that bigenotypic rearrangements characterize some T-cell LBLs. We conclude that FNA samples are adequate for accurate characterization of LBL and may obviate the need for surgical biopsy.
Original language | English (US) |
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Pages (from-to) | 887-894 |
Number of pages | 8 |
Journal | Acta Cytologica |
Volume | 36 |
Issue number | 6 |
State | Published - 1992 |
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology