FK506 as an adjuvant of tolerogenic DNA vaccination for the prevention of experimental autoimmune encephalomyelitis

Youmin Kang, Jia Zhao, Yue Liu, Aoshuang Chen, Guoxing Zheng, Yang Yu, Jianjie Mi, Qiang Zou, Bin Wang

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13 Scopus citations

Abstract

Background: DNA vaccination is a strategy that has been developed primarily to elicit protective immunity against infection and cancer. Methods: DNA vaccine was used, in conjunction with an immunosuppressant, to tolerize harmful autoimmunity. Results: Immunization of C57BL/6 mice with MOG35-55, a myelin oligodendrocyte glycoprotein-derived peptide, and FK506 (Tacrolimus) as a tolerogenic adjuvant stimulated regulatory dendritic cells, induced antigenspecific regulatory T cells (Treg), and protected the animals from subsequent induction of experimental autoimmune encephalomyelitis (EAE). After EAE induction, there were fewer lymphocytes, including fewer T helper 17 cells, and more Treg infiltrating the spinal cord in the immunized mice compared to in control mice. Furthermore, at the peak of the EAE manifestation, CD4 T cells in the immunized mice showed decreased expression of interferon-γ and interleukin (IL)-17, but not IL-4, in treated mice. Conclusions: DNA vaccination, when applied with an immunosuppressant as adjuvant, can induce antigen-specific tolerance and prevent autoimmune disease.

Original languageEnglish (US)
Pages (from-to)1064-1070
Number of pages7
JournalJournal of Gene Medicine
Volume11
Issue number11
DOIs
StatePublished - Dec 1 2009

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Keywords

  • Adjuvant
  • DNA vaccination
  • EAE
  • FK506
  • Prevention
  • Tolerance

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Drug Discovery
  • Genetics(clinical)

Cite this

Kang, Y., Zhao, J., Liu, Y., Chen, A., Zheng, G., Yu, Y., Mi, J., Zou, Q., & Wang, B. (2009). FK506 as an adjuvant of tolerogenic DNA vaccination for the prevention of experimental autoimmune encephalomyelitis. Journal of Gene Medicine, 11(11), 1064-1070. https://doi.org/10.1002/jgm.1387