Abstract
Processing biological samples by solubilization in Hyamine hydroxide (methylbenzethonium hydroxide) as an alternative to wet ashing with concentrated nitric acid for atomic absorption determination of platinum has been explored. The concentrations of platinum in tissues removed from rats 2 h after they had received the antitumor drug Carboplatin (60 mg/kg, iv) were comparable using either of the procedures, indicating the utility of tissue solubilization. Limits of detection were also comparable between the Hyamine hydroxide (0.25 μg platinum/g) and nitric acid (0.15 μg/g) procedures. The solubilization method, however, has advantages over wet digestion because of its simplicity and greater safety.
Original language | English (US) |
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Pages (from-to) | 21-26 |
Number of pages | 6 |
Journal | Analytical Biochemistry |
Volume | 163 |
Issue number | 1 |
DOIs | |
State | Published - May 15 1987 |
Externally published | Yes |
Keywords
- Carboplatin
- atomic absorption spectroscopy
- cancer chemotherapy
- platinum determination
- tissue distribution
- tissue solubilization
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology