Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases

Prashant R. Tembhare; Constance M. Yuan; David Venzon; Raul Braylan; Neha Korde; Elisabet Manasanch; Diamond Zuchlinsky; Katherine Calvo; Roger Kurlander; Manisha Bhutani; Nishant Tageja; Irina Maric; Marcia Mulquin; Mark Roschewski; Mary Kwok; David Liewehr; Ola Landgren; Maryalice Stetler-Stevenson

doi:10.1016/j.leukres.2013.12.007

Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases

Prashant R. Tembhare, Constance M. Yuan, David Venzon, Raul Braylan, Neha Korde, Elisabet Manasanch, Diamond Zuchlinsky, Katherine Calvo, Roger Kurlander, Manisha Bhutani, Nishant Tageja, Irina Maric, Marcia Mulquin, Mark Roschewski, Mary Kwok, David Liewehr, Ola Landgren, Maryalice Stetler-Stevenson

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N= 34) and APC in a series of unselected PCD (N= 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N= 14), SMM ( N= 35) and MM (N= 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p= 0.0002).

Original language	English (US)
Pages (from-to)	371-376
Number of pages	6
Journal	Leukemia Research
Volume	38
Issue number	3
DOIs	https://doi.org/10.1016/j.leukres.2013.12.007
State	Published - Mar 2014
Externally published	Yes

Keywords

CD81
Monoclonal gammopathy of uncertain significance
Multiple myeloma
Normal plasma cell immunophenotype
Smoldering multiple myeloma

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/j.leukres.2013.12.007

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Tembhare, P. R., Yuan, C. M., Venzon, D., Braylan, R., Korde, N., Manasanch, E., Zuchlinsky, D., Calvo, K., Kurlander, R., Bhutani, M., Tageja, N., Maric, I., Mulquin, M., Roschewski, M., Kwok, M., Liewehr, D., Landgren, O., & Stetler-Stevenson, M. (2014). Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases. Leukemia Research, 38(3), 371-376. https://doi.org/10.1016/j.leukres.2013.12.007

Tembhare, PR, Yuan, CM, Venzon, D, Braylan, R, Korde, N, Manasanch, E, Zuchlinsky, D, Calvo, K, Kurlander, R, Bhutani, M, Tageja, N, Maric, I, Mulquin, M, Roschewski, M, Kwok, M, Liewehr, D, Landgren, O & Stetler-Stevenson, M 2014, 'Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases', Leukemia Research, vol. 38, no. 3, pp. 371-376. https://doi.org/10.1016/j.leukres.2013.12.007

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title = "Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases",

abstract = "Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N= 34) and APC in a series of unselected PCD (N= 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N= 14), SMM ( N= 35) and MM (N= 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p= 0.0002).",

keywords = "CD81, Monoclonal gammopathy of uncertain significance, Multiple myeloma, Normal plasma cell immunophenotype, Smoldering multiple myeloma",

author = "Tembhare, {Prashant R.} and Yuan, {Constance M.} and David Venzon and Raul Braylan and Neha Korde and Elisabet Manasanch and Diamond Zuchlinsky and Katherine Calvo and Roger Kurlander and Manisha Bhutani and Nishant Tageja and Irina Maric and Marcia Mulquin and Mark Roschewski and Mary Kwok and David Liewehr and Ola Landgren and Maryalice Stetler-Stevenson",

note = "Funding Information: This research was funded by the intramural program of the National Cancer Institute , National Institutes of Health . The authors wish to acknowledge the technical assistance of Catharine McCoy, Gregory Jasper and Linda Weaver. ",

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doi = "10.1016/j.leukres.2013.12.007",

language = "English (US)",

volume = "38",

pages = "371--376",

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AU - Tembhare, Prashant R.

AU - Yuan, Constance M.

AU - Venzon, David

AU - Braylan, Raul

AU - Korde, Neha

AU - Manasanch, Elisabet

AU - Zuchlinsky, Diamond

AU - Calvo, Katherine

AU - Kurlander, Roger

AU - Bhutani, Manisha

AU - Tageja, Nishant

AU - Maric, Irina

AU - Mulquin, Marcia

AU - Roschewski, Mark

AU - Kwok, Mary

AU - Liewehr, David

AU - Landgren, Ola

AU - Stetler-Stevenson, Maryalice

N1 - Funding Information: This research was funded by the intramural program of the National Cancer Institute , National Institutes of Health . The authors wish to acknowledge the technical assistance of Catharine McCoy, Gregory Jasper and Linda Weaver.

PY - 2014/3

Y1 - 2014/3

N2 - Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N= 34) and APC in a series of unselected PCD (N= 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N= 14), SMM ( N= 35) and MM (N= 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p= 0.0002).

AB - Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N= 34) and APC in a series of unselected PCD (N= 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N= 14), SMM ( N= 35) and MM (N= 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p= 0.0002).

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KW - Monoclonal gammopathy of uncertain significance

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KW - Normal plasma cell immunophenotype

KW - Smoldering multiple myeloma

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Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases

Abstract

Keywords

ASJC Scopus subject areas

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