TY - JOUR
T1 - Formation of the Cross-Linked Base, Diguanylethane, in DNA Treated with N, N’-Bis(2-Chloroethyl)-N-Nitrosourea
AU - Tong, William P.
AU - Ludlum, David B.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1981/2/1
Y1 - 1981/2/1
N2 - A cross-linked base, diguanylethane, has been isolated and identified as a product of the reaction of N, N’-bis(2-chloro-ethyl)-N-nitrosourea with DNA. DNA was reacted at 37° with N, N’-bis(2-chloroethyl)-N-nitosourea in neutral aqueous solution, isolated by precipitation with ethanol, washed, and heated briefly at 100° to release a purine fraction. This fraction was separated by high-pressure liquid chromatography and found to contain both chloroethylguanine and 1,2-diguanyl-ethane. Formation of the latter product may be related to the cytotoxicity of N, N’-bis(2-chloroethyl)-N-nitrosourea.
AB - A cross-linked base, diguanylethane, has been isolated and identified as a product of the reaction of N, N’-bis(2-chloro-ethyl)-N-nitrosourea with DNA. DNA was reacted at 37° with N, N’-bis(2-chloroethyl)-N-nitosourea in neutral aqueous solution, isolated by precipitation with ethanol, washed, and heated briefly at 100° to release a purine fraction. This fraction was separated by high-pressure liquid chromatography and found to contain both chloroethylguanine and 1,2-diguanyl-ethane. Formation of the latter product may be related to the cytotoxicity of N, N’-bis(2-chloroethyl)-N-nitrosourea.
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M3 - Article
C2 - 7448781
AN - SCOPUS:0019363929
SN - 0008-5472
VL - 41
SP - 380
EP - 382
JO - Cancer Research
JF - Cancer Research
IS - 2
ER -