TY - JOUR
T1 - Formulation and toxicity of liposomes containing rifampicin
AU - Constantino, Luis
AU - Mehta, Reeta T.
AU - Cruz, M. Eugénia
AU - Lopez-Berestein, Gabriel
N1 - Funding Information:
Luis Constantino is a recipient of a grant from JNICT (Portugal). This project was supported in part by a grant of Fundacao Luso Americana para o Desenvolvimento (FLAD) to M. Eugenia M. Cruz. We thank Manuela Gaspar for excellent technical assistance and M. Luisa Menezes for typing the manuscript.
PY - 1993
Y1 - 1993
N2 - RFP was incorporated in liposomes and the effect of lipid composition on incorporation of drug and stability of the vesicles was studied. The amount of RFP incorporated is dependent on the lipid composition, being higher when charged lipids were used. RFP incorporation increased as the fluidity of the vesicles increased. Stability in saline was related to liposomes with high encapsulation of RFP, while stability in serum was superior when phospholipids with a Tc higher than 37°C were used. In vitro RFP and L-RFP were not toxic to erythrocytes; however, L-RFP was less toxic than RFP to CHO cells and to macrophages. In concentrations up to 340 mg x Kg-1, L-RFP was not toxic to mice.
AB - RFP was incorporated in liposomes and the effect of lipid composition on incorporation of drug and stability of the vesicles was studied. The amount of RFP incorporated is dependent on the lipid composition, being higher when charged lipids were used. RFP incorporation increased as the fluidity of the vesicles increased. Stability in saline was related to liposomes with high encapsulation of RFP, while stability in serum was superior when phospholipids with a Tc higher than 37°C were used. In vitro RFP and L-RFP were not toxic to erythrocytes; however, L-RFP was less toxic than RFP to CHO cells and to macrophages. In concentrations up to 340 mg x Kg-1, L-RFP was not toxic to mice.
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U2 - 10.3109/08982109309148215
DO - 10.3109/08982109309148215
M3 - Article
AN - SCOPUS:0027217238
SN - 0898-2104
VL - 3
SP - 275
EP - 301
JO - Journal of Liposome Research
JF - Journal of Liposome Research
IS - 2
ER -