TY - JOUR
T1 - Fragment complementation for the co-refolding of Thermotoga maritima β-glucosidase by gene splitting at non-homologous region
AU - Kim, Bong Jo
AU - Mangala, Selanere L.
AU - Muralidhara, B. K.
AU - Hayashi, Kiyoshi
N1 - Funding Information:
Bong-Jo Kim and S.L. Mangala thank the Japan Science and Technology Corporation (JST) for the award of an STA fellowship. This work is supported in part by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences in Japan.
PY - 2007/3/5
Y1 - 2007/3/5
N2 - β-Glucosidase from Thermotoga maritima is a 721 amino acid protein consisting of structurally distinct non-homologous region connecting the N- and C-terminal domains. To investigate the functional role of the non-homologous region in co-refolding, the gene was split at four sites (370, 403, 419 and 435) of the non-homologous region, cloned and separately expressed in E. coli generating eight peptide fragments (N370, N403, N419, N435, 371C, 404C, 420C and 436C). All eight fragments were recovered as insoluble inclusion bodies and found to be catalytically inactive. No catalytic activity was observed when these eight fragments were refolded individually. However, the catalytic activity was recovered when the two fragments derived from N- and C-terminal peptides were co-refolded together. Truncation of almost all amino acid residues in non-homologous region resulted in extremely low catalytic activity, which strongly suggests that non-homologous region is very important for the proper refolding of the peptides to reconstitute the catalytic activity. We succeeded in refolding the protein into an active form after splitting the gene at non-homologous region, denaturing and co-refolding the two domains. These results demonstrates the importance of structural elements that are not involved in the active site play an important role in protein folding to assemble the active site elements.
AB - β-Glucosidase from Thermotoga maritima is a 721 amino acid protein consisting of structurally distinct non-homologous region connecting the N- and C-terminal domains. To investigate the functional role of the non-homologous region in co-refolding, the gene was split at four sites (370, 403, 419 and 435) of the non-homologous region, cloned and separately expressed in E. coli generating eight peptide fragments (N370, N403, N419, N435, 371C, 404C, 420C and 436C). All eight fragments were recovered as insoluble inclusion bodies and found to be catalytically inactive. No catalytic activity was observed when these eight fragments were refolded individually. However, the catalytic activity was recovered when the two fragments derived from N- and C-terminal peptides were co-refolded together. Truncation of almost all amino acid residues in non-homologous region resulted in extremely low catalytic activity, which strongly suggests that non-homologous region is very important for the proper refolding of the peptides to reconstitute the catalytic activity. We succeeded in refolding the protein into an active form after splitting the gene at non-homologous region, denaturing and co-refolding the two domains. These results demonstrates the importance of structural elements that are not involved in the active site play an important role in protein folding to assemble the active site elements.
KW - Co-refolding
KW - Fragment complementation
KW - Gene splitting
KW - Inclusion bodies
KW - Non-homologous region
KW - Thermotoga maritima
KW - β-Glucosidase
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U2 - 10.1016/j.enzmictec.2006.06.005
DO - 10.1016/j.enzmictec.2006.06.005
M3 - Article
AN - SCOPUS:33847281822
SN - 0141-0229
VL - 40
SP - 732
EP - 739
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 4
ER -