Abstract
This report describes a highly reproducible single cell cytotoxic assay which can measure the frequency of K cells in human peripheral blood lymphocytes. This assay is based on the ability of FcR-bearing lymphocytes to bind to antibody sensitized target cells via their FcR. Approximately 30% of lymphocytes which bound antibody sensitized P815 cells (P815-AB) were K cells. Cytolysis of the conjugated P815-AB fulfilled all the criteria for ADCC. Furthermore, an excellent correlation was observed between the frequency of K cells and % cytotoxicity as measured by the ADCC 51Cr-release assay. The mean frequency of human K cells in peripheral blood lymphocytes from 20 volunteers was measured to be 3.2% of the total lymphocyte populations
Original language | English (US) |
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Pages (from-to) | 255-268 |
Number of pages | 14 |
Journal | Journal of Immunological Methods |
Volume | 36 |
Issue number | 3-4 |
DOIs | |
State | Published - 1980 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology