Abstract
Since last decade, biogenic synthesis of metal or metal-oxide nanoparticles is emerging as an alternative method, which is environment friendly, simple and safe to use. In this article, fruit waste (peel) extract (FWE) of three citrus fruits viz. Citrus limon, Citrus sinensis, and Citrus limetta were used as bio-reductant for green and sustainable synthesis of silver nanoparticles (AgNPs). As-synthesised AgNPs were characterized by using UV–vis spectroscopy, Dynamic light scattering, and High Resolution Transmission Electron Microscopy. TEM studies revealed 9–46 nm size range of synthesized AgNPs. The antimicrobial and antioxidant activities were also studied by using Agar well diffusion method and DPPH Assay, respectively. Nanoparticles showed good antimicrobial activity against both Gram positive (S. aureus) and Gram negative (E. coli) bacteria. Further, bioactivity assays revealed selective cytotoxicity (anticancer) of the nanoparticles against human lung cancer cell line A549. The nanoparticles are able to induce cancer cell specific apoptosis at G0/G1 phase of cell cycle. The results showed potential mechanism of action of nanoparticles via augmentation of antioxidant system in cancer cells. Over all, this study show multifaceted potential bioactivities of nanoparticles generated from fruit waste.
Original language | English (US) |
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Pages (from-to) | 221-231 |
Number of pages | 11 |
Journal | Journal of Applied Biomedicine |
Volume | 16 |
Issue number | 3 |
DOIs | |
State | Published - Aug 2018 |
Externally published | Yes |
Keywords
- Anticancer
- Antimicrobial
- Antioxidant
- Biosynthesis
- Citrus fruits
- Silver nanoparticles
ASJC Scopus subject areas
- General Medicine
- Biomedical Engineering
- General Immunology and Microbiology
- Health, Toxicology and Mutagenesis
- Artificial Intelligence