@article{a23f3f6282ee4ba685ad881f9b53a004,
title = "Functional genetic analysis of mouse chromosome 11",
abstract = "Now that the mouse and human genome sequences are complete, biologists need systematic approaches to determine the function of each gene. A powerful way to discover gene function is to determine the consequence of mutations in living organisms. Large-scale production of mouse mutations with the point mutagen N-ethyl-N-nitrosourea (ENU) is a key strategy for analysing the human genome because mouse mutants will reveal functions unique to mammals, and many may model human diseases. To examine genes conserved between human and mouse, we performed a recessive ENU mutagenesis screen that uses a balancer chromosome, inversion chromosome 11 (refs 4, 5). Initially identified in the fruitfly, balancer chromosomes are valuable genetic tools that allow the easy isolation of mutations on selected chromosomes. Here we show the isolation of 230 new recessive mouse mutations, 88 of which are on chromosome 11. This genetic strategy efficiently generates and maps mutations on a single chromosome, even as mutations throughout the genome are discovered. The mutations reveal new defects in haematopoiesis, craniofacial and cardiovascular development, and fertility.",
author = "Kile, {Benjamin T.} and Hentges, {Kathryn E.} and Clark, {Amander T.} and Hisashi Nakamura and Salinger, {Andrew P.} and Bin Liu and Nell Box and Stockton, {David W.} and Johnson, {Randy L.} and Behringer, {Richard R.} and Allan Bradley and Justice, {Monica J.}",
note = "Funding Information: Acknowledgements We thank R. Grosberg, S. Nee, A. Griffin, D. Shuker and M. Stanton for comments, P. Graham, D. Phillips and M. King for advice on methods, R. Nelson and D. Smith for soybean cultivars {\textquoteleft}T243{\textquoteright} and {\textquoteleft}S0066{\textquoteright}, and P. van Berkum for B. japonicum 110ARS. This work was supported by the NSF (grant to R.F.D. and graduate fellowship to E.T.K.), the California Agricultural Experiment Station, the Land Institute, the Royal Society, the BBSRC and the NERC. Funding Information: Acknowledgements We thank C. Viator, C. Dinh, S. Moncrief, A. Zalud, J. Maffucci, C. Mason-Garrison, K. Firozi, M. Alviento, C. Hubbard, B. Hasson and M. Scantlin for technical assistance, and J. Zhong and M. Patterson for database support. We also thank L. Peters for the gift of Slc4a1 knockout mice. Y. Furuta, H. Bellen, S. Lovell, S. Watowich and H. Gilbert are thanked for critical reading of this manuscript. This work was supported by NIH grants to M.J.J. and A.B. K.E.H. was supported by an NIH-NRSA grant. B.T.K. is a Fellow of the Leukemia Research Foundation.",
year = "2003",
month = sep,
day = "4",
doi = "10.1038/nature01865",
language = "English (US)",
volume = "425",
pages = "81--86",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6953",
}