TY - JOUR
T1 - Fxr1p but not fmrp regulates the levels of mammalian brain-specific microrna-9 and microrna-124
AU - Xu, Xia Lian
AU - Zong, Ruiting
AU - Li, Zhaodong
AU - Biswas, Md Helal Uddin
AU - Fang, Zhe
AU - Nelson, David L.
AU - Gao, Fen Biao
PY - 2011/9/28
Y1 - 2011/9/28
N2 - Mammalian brain-specific miR-9 and miR-124 have been implicated in several aspects of neuronal development and function. However, it is not known how their expression levels are regulated in vivo.We found that the levels of miR-9 and miR-124 are regulated by FXR1P but not by the loss of FXR2P or FMRP in vivo, a mouse model of fragile X syndrome. Surprisingly, the levels of miR-9 and miR-124 are elevated in fmr1/fxr2 double-knock-out mice, in part reflecting posttranscriptional upregulation of FXR1P. Indeed, FXR1P is required for efficient processing of pre-miR-9 and pre-miR-124 in vitro and forms a complex with Dicer and pre-miRNAs. These findings reveal differential roles of FMRP family proteins in controlling the expression levels of brain-specific miRNAs.
AB - Mammalian brain-specific miR-9 and miR-124 have been implicated in several aspects of neuronal development and function. However, it is not known how their expression levels are regulated in vivo.We found that the levels of miR-9 and miR-124 are regulated by FXR1P but not by the loss of FXR2P or FMRP in vivo, a mouse model of fragile X syndrome. Surprisingly, the levels of miR-9 and miR-124 are elevated in fmr1/fxr2 double-knock-out mice, in part reflecting posttranscriptional upregulation of FXR1P. Indeed, FXR1P is required for efficient processing of pre-miR-9 and pre-miR-124 in vitro and forms a complex with Dicer and pre-miRNAs. These findings reveal differential roles of FMRP family proteins in controlling the expression levels of brain-specific miRNAs.
UR - http://www.scopus.com/inward/record.url?scp=80053226174&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053226174&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.2827-11.2011
DO - 10.1523/JNEUROSCI.2827-11.2011
M3 - Article
C2 - 21957233
AN - SCOPUS:80053226174
SN - 0270-6474
VL - 31
SP - 13705
EP - 13709
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 39
ER -