Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids

Isik G. Yulug; Ann M. Killary; Arbansjit K. Sandhu; Raghbir S. Athwal; Margaret Fox; Elizabeth M.C. Fisher

doi:10.1007/BF02257471

Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids

Isik G. Yulug, Ann M. Killary, Arbansjit K. Sandhu, Raghbir S. Athwal, Margaret Fox, Elizabeth M.C. Fisher

Research output: Contribution to journal › Article › peer-review

Abstract

To access a wide variety of expressed sequences from human chromosome 21 we have placed this chromosome into undifferentiated P19 mouse embryonic carcinoma cells. Cell lines resulting from these experiments have a range of morphologies and a wide variety of karyotypes. We have studied the retinoic acid response of five cell lines, compared to P19 cells, by observing three markers of retinoic acid induced P19 differentiation-cell morphology, RARα and Wnt1 transcription. We see an 'early' retinoic acid response effect, however this response breaks down by the time the 'late' gene Wnt1 would be transcribed in P19 cells. A highly responsive cell line will be useful for cloning expressed sequences from human chromosome 21 which are produced by early genes in retinoic acid inducible pathways, such as those involved in neurogenesis.

Original language	English (US)
Pages (from-to)	357-365
Number of pages	9
Journal	Somatic Cell and Molecular Genetics
Volume	21
Issue number	5
DOIs	https://doi.org/10.1007/BF02257471
State	Published - Sep 1995

ASJC Scopus subject areas

Genetics
Cell Biology

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title = "Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids",

abstract = "To access a wide variety of expressed sequences from human chromosome 21 we have placed this chromosome into undifferentiated P19 mouse embryonic carcinoma cells. Cell lines resulting from these experiments have a range of morphologies and a wide variety of karyotypes. We have studied the retinoic acid response of five cell lines, compared to P19 cells, by observing three markers of retinoic acid induced P19 differentiation-cell morphology, RARα and Wnt1 transcription. We see an 'early' retinoic acid response effect, however this response breaks down by the time the 'late' gene Wnt1 would be transcribed in P19 cells. A highly responsive cell line will be useful for cloning expressed sequences from human chromosome 21 which are produced by early genes in retinoic acid inducible pathways, such as those involved in neurogenesis.",

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AU - Yulug, Isik G.

AU - Killary, Ann M.

AU - Sandhu, Arbansjit K.

AU - Athwal, Raghbir S.

AU - Fox, Margaret

AU - Fisher, Elizabeth M.C.

PY - 1995/9

Y1 - 1995/9

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AB - To access a wide variety of expressed sequences from human chromosome 21 we have placed this chromosome into undifferentiated P19 mouse embryonic carcinoma cells. Cell lines resulting from these experiments have a range of morphologies and a wide variety of karyotypes. We have studied the retinoic acid response of five cell lines, compared to P19 cells, by observing three markers of retinoic acid induced P19 differentiation-cell morphology, RARα and Wnt1 transcription. We see an 'early' retinoic acid response effect, however this response breaks down by the time the 'late' gene Wnt1 would be transcribed in P19 cells. A highly responsive cell line will be useful for cloning expressed sequences from human chromosome 21 which are produced by early genes in retinoic acid inducible pathways, such as those involved in neurogenesis.

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Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids

Abstract

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