TY - JOUR
T1 - Gene-targeting strategies.
AU - Cheah, S. S.
AU - Behringer, R. R.
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2000
Y1 - 2000
N2 - Gene targeting in mouse ES cells is a powerful method for studying gene function in vivo. For the novice, this combination of molecular biology, specialized tissue-culture cell lines, and mouse reproductive biology can be daunting. We present a straightforward, one might say constrained, guide for novices of gene targeting to generate a null allele in large or small protein-coding genes. The method we outline has evolved from years of experience of training and advising beginners on this powerful technology. We believe that a good design for a gene-targeting strategy ultimately saves time, money, and research animal lives. Once you feel comfortable with a fundamental knockout, we suggest you consider the new and exciting gene-targeting variations that can be used to address important biological questions (2).
AB - Gene targeting in mouse ES cells is a powerful method for studying gene function in vivo. For the novice, this combination of molecular biology, specialized tissue-culture cell lines, and mouse reproductive biology can be daunting. We present a straightforward, one might say constrained, guide for novices of gene targeting to generate a null allele in large or small protein-coding genes. The method we outline has evolved from years of experience of training and advising beginners on this powerful technology. We believe that a good design for a gene-targeting strategy ultimately saves time, money, and research animal lives. Once you feel comfortable with a fundamental knockout, we suggest you consider the new and exciting gene-targeting variations that can be used to address important biological questions (2).
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U2 - 10.1385/1-59259-065-9:455
DO - 10.1385/1-59259-065-9:455
M3 - Review article
C2 - 10840733
AN - SCOPUS:0033658231
SN - 1064-3745
VL - 136
SP - 455
EP - 463
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -