Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain

David W. Threadgill; Della Yee; Argabin Matin; Joseph H. Nadeau; Terry Magnuson

doi:10.1007/s003359900453

Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain

David W. Threadgill, Della Yee, Argabin Matin, Joseph H. Nadeau, Terry Magnuson

Genetics

Research output: Contribution to journal › Article › peer-review

189 Scopus citations

Abstract

The 129 mouse is the most widely used strain in gene targeting experiments. However, numerous substrains exist with demonstrable physiological differences. In this study a set of simple sequence length polymorphisms (SSLPs) was used to determine the relatedness of selected 129 substrains. 129/SvJ was significantly different from the other 129 substrains and is more accurately classified as a recombinant congenic strain (129cX/Sv), being derived from 129/Sv and an unknown strain. This mixed genetic background could complicate gene targeting experiments by reducing homologous recombination efficiency when constructs and ES cells are not derived from the same 129 substrain. Additionally, discrepancies due to different genetic backgrounds may arise when comparing phenotypes of genes targeted in different 129-derived ES cell lines.

Original language	English (US)
Pages (from-to)	390-393
Number of pages	4
Journal	Mammalian Genome
Volume	8
Issue number	6
DOIs	https://doi.org/10.1007/s003359900453
State	Published - Jun 1997

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Genetics

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abstract = "The 129 mouse is the most widely used strain in gene targeting experiments. However, numerous substrains exist with demonstrable physiological differences. In this study a set of simple sequence length polymorphisms (SSLPs) was used to determine the relatedness of selected 129 substrains. 129/SvJ was significantly different from the other 129 substrains and is more accurately classified as a recombinant congenic strain (129cX/Sv), being derived from 129/Sv and an unknown strain. This mixed genetic background could complicate gene targeting experiments by reducing homologous recombination efficiency when constructs and ES cells are not derived from the same 129 substrain. Additionally, discrepancies due to different genetic backgrounds may arise when comparing phenotypes of genes targeted in different 129-derived ES cell lines.",

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AU - Threadgill, David W.

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AU - Magnuson, Terry

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AB - The 129 mouse is the most widely used strain in gene targeting experiments. However, numerous substrains exist with demonstrable physiological differences. In this study a set of simple sequence length polymorphisms (SSLPs) was used to determine the relatedness of selected 129 substrains. 129/SvJ was significantly different from the other 129 substrains and is more accurately classified as a recombinant congenic strain (129cX/Sv), being derived from 129/Sv and an unknown strain. This mixed genetic background could complicate gene targeting experiments by reducing homologous recombination efficiency when constructs and ES cells are not derived from the same 129 substrain. Additionally, discrepancies due to different genetic backgrounds may arise when comparing phenotypes of genes targeted in different 129-derived ES cell lines.

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Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain

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