Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase

Cheng Chi Lee; Xiangwei Wu; Richard A. Gibbs; Richard G. Cook; Donna M. Muzny; C. Thomas Caskey

doi:10.1126/science.3344434

Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase

Cheng Chi Lee, Xiangwei Wu, Richard A. Gibbs, Richard G. Cook, Donna M. Muzny, C. Thomas Caskey

Clinical Cancer Prevention

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Abstract

Urate oxidase (E.C. 1.7.3.3) catalyzes the oxidation of uric acid to allantoin in most mammals except humans and certain primates. The amino-terminal amino acid sequence for porcine urate oxidase was determined and used in a novel procedure for generating complementary DNA (cDNA) probes to this amino acid sequence. The procedure is based on the polymerase chain reaction and utilizes mixed oligonucleotide primers complementary to the reverse translation products of an amino acid sequence. This rapid and simple cDNA cloning procedure is generally applicable and requires only a partial amino acid sequence. A cDNA probe developed by this procedure was used to isolate a full-length porcine urate oxidase cDNA and to demonstrate the presence of homologous genomic sequences in humans.

Original language	English (US)
Pages (from-to)	1288-1291
Number of pages	4
Journal	Science
Volume	239
Issue number	4846
DOIs	https://doi.org/10.1126/science.3344434
State	Published - 1988

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title = "Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase",

abstract = "Urate oxidase (E.C. 1.7.3.3) catalyzes the oxidation of uric acid to allantoin in most mammals except humans and certain primates. The amino-terminal amino acid sequence for porcine urate oxidase was determined and used in a novel procedure for generating complementary DNA (cDNA) probes to this amino acid sequence. The procedure is based on the polymerase chain reaction and utilizes mixed oligonucleotide primers complementary to the reverse translation products of an amino acid sequence. This rapid and simple cDNA cloning procedure is generally applicable and requires only a partial amino acid sequence. A cDNA probe developed by this procedure was used to isolate a full-length porcine urate oxidase cDNA and to demonstrate the presence of homologous genomic sequences in humans.",

author = "Lee, {Cheng Chi} and Xiangwei Wu and Gibbs, {Richard A.} and Cook, {Richard G.} and Muzny, {Donna M.} and {Thomas Caskey}, C.",

year = "1988",

doi = "10.1126/science.3344434",

language = "English (US)",

volume = "239",

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TY - JOUR

T1 - Generation of cDNA Probes Directed by Amino Acid Sequence

T2 - Cloning of Urate Oxidase

AU - Lee, Cheng Chi

AU - Wu, Xiangwei

AU - Gibbs, Richard A.

AU - Cook, Richard G.

AU - Muzny, Donna M.

AU - Thomas Caskey, C.

PY - 1988

Y1 - 1988

N2 - Urate oxidase (E.C. 1.7.3.3) catalyzes the oxidation of uric acid to allantoin in most mammals except humans and certain primates. The amino-terminal amino acid sequence for porcine urate oxidase was determined and used in a novel procedure for generating complementary DNA (cDNA) probes to this amino acid sequence. The procedure is based on the polymerase chain reaction and utilizes mixed oligonucleotide primers complementary to the reverse translation products of an amino acid sequence. This rapid and simple cDNA cloning procedure is generally applicable and requires only a partial amino acid sequence. A cDNA probe developed by this procedure was used to isolate a full-length porcine urate oxidase cDNA and to demonstrate the presence of homologous genomic sequences in humans.

AB - Urate oxidase (E.C. 1.7.3.3) catalyzes the oxidation of uric acid to allantoin in most mammals except humans and certain primates. The amino-terminal amino acid sequence for porcine urate oxidase was determined and used in a novel procedure for generating complementary DNA (cDNA) probes to this amino acid sequence. The procedure is based on the polymerase chain reaction and utilizes mixed oligonucleotide primers complementary to the reverse translation products of an amino acid sequence. This rapid and simple cDNA cloning procedure is generally applicable and requires only a partial amino acid sequence. A cDNA probe developed by this procedure was used to isolate a full-length porcine urate oxidase cDNA and to demonstrate the presence of homologous genomic sequences in humans.

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Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase

Abstract

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