TY - JOUR
T1 - Genetic Contamination of an SJL/J Mouse Colony
T2 - Rapid Detection by PCR-based Microsatellite Analysis
AU - Benavides, Fernando J.
N1 - Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
PY - 1999/3
Y1 - 1999/3
N2 - Six simple sequence-length polymorphisms (SSLPs), or microsatellites, generated by polymerase chain reaction (PCR), were used to detect genetic contamination of an SJL/J mouse colony in a faster and more economical way than standard immunologic and biochemical markers. The marker set was chosen to assess SJL authenticity and to distinguish a possible outcross with BALB/c, the only other albino strain maintained at the facility. Evidence of genetic contamination as a result of an outcross with BALB/c was found, and the results were ready within a workday.
AB - Six simple sequence-length polymorphisms (SSLPs), or microsatellites, generated by polymerase chain reaction (PCR), were used to detect genetic contamination of an SJL/J mouse colony in a faster and more economical way than standard immunologic and biochemical markers. The marker set was chosen to assess SJL authenticity and to distinguish a possible outcross with BALB/c, the only other albino strain maintained at the facility. Evidence of genetic contamination as a result of an outcross with BALB/c was found, and the results were ready within a workday.
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M3 - Article
AN - SCOPUS:0002710098
SN - 1060-0558
VL - 38
SP - 54
EP - 55
JO - Contemporary Topics in Laboratory Animal Science
JF - Contemporary Topics in Laboratory Animal Science
IS - 2
ER -